Study on the response surface optimization of extraction technology of total triterpenes from Forsythia suspensa leaves and its anti-tumor activity

doi:10.14088/j.cnki.issn0439-8114.2023.09.021

Abstract

Abstract: Taking the extraction rate of total triterpenes as the evaluation indicator, the optimum extraction technology of total triterpenes from leaves of Forsythia suspensa was optimized by ultrasonic extraction technology combined with single factor test and response surface test. Six kinds of macroporous resins were used for further purification, and CCK-8 method was used to detect the proliferation inhibitory effect of total triterpenes of Forsythia suspensa leaves on the human breast cancer MCF-7 and MDA-MB-231 cells, liver cancer HepG2 and SNU-739 cells, colon cancer HT29 and HCT116 cells. The results showed that the optimum extraction condition of total triterpenes from Forsythia suspensa leaves was ethanol concentration of 85%, ratio of material to liquid of 1∶40 g/mL, extraction time of 35 min, and extraction temperature of 60 ℃. Under this condition, the extraction rate of total triterpenes from Forsythia suspensa leaves could reach 14.13%. D101 resin was the best resin, and the content of total triterpenes from Forsythia suspensa leaves could reach 72.89%. The 50% inhibitory concentration （IC₅₀） of total triterpenes from Forsythia suspensa leaves to MCF-7, MDA-MB-231, HepG2, SNU-739, HT29 and HCT116 cells was （6.29±0.34）, （9.51±0.71）, （12.05±1.23）, （9.91±0.94）, （22.82 ±0.65）, and （23.45 ±1.01） mg/L, respectively. The optimized extraction process for total triterpenes from Forsythia suspensa leaves was stable and simple, and it had strong anti-tumor activity after purification.

Key words: Forsythia suspensa leaves, total triterpenes, response surface, extraction, purification, anti-tumor

CLC Number:

R284.2

WANG Xue-fang, CHEN Ling, GUI Rong, NING Er-juan, WANG Wei, WANG Xue-bing, LI Xiao. Study on the response surface optimization of extraction technology of total triterpenes from Forsythia suspensa leaves and its anti-tumor activity[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(9): 113-118.

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