Abstract: The factors and levels of response surface design were determined by the single factor test, and the extraction rate was used as the evaluation index. Combined with Box-Behnken design principle and response surface analysis method, the optimal extraction process of essential oil from Pistacia weinmannifolia leaves was determined. The results showed that the optimal extraction process of Pistacia weinmannifolia leaves was soaking for 2.23 h, adding 10.93 times of the amount of water to the medicinal materials, and extracting for 5.22 h. Under this condition, the extraction rate was 0.481 2%, which was the optimal condition to investigate the factors. The optimized process was simple and stable, and it provided a feasible scheme for the extraction of essential oil from Pistacia weinmannifolia leaves.

Key words: Pistacia weinmannifolia leaves, volatile oil, single factor extraction, response surface analysis