Determination of the concentration of flue-cured tobacco volatile oils by UV-spectrophotography

doi:10.14088/j.cnki.issn0439-8114.2020.18.023

Abstract

Abstract: To determining the concentration of volatile oils solution extracted from flue-cured tobacco leaves, a method based on UV spectrophotometry was researched. Volatile oils were extracted by simultaneous distillation and diluted with dicholormethane to prepare a set of gradient solutions. Each solution was scanned by UV spectrophotomet. The best working wavelength was selected to establish linear regression equation between the concentration of volatile oil and its ultraviolet absorbance. Results showed that the volatile oils had a large absorption spectrum between 225~245 nm. The best analysis wavelength was 239 nm, the linear relationship was y =0.250 1x + 0.039 3, R²=0.999 3. Its relative error of was less than 0.5%, the limit of detection （LOD） and the limit of quantification （LOQ） of the working curve were 0.153 g/L and 0.464 g/L separately.

Key words: flue-cured tobacco, volatile oils, ultraviolet light, spectrophotography, determination

CLC Number:

O69
S572

WANG Ke-han, XIANG Huan, YANG Yi-de, ZHANG Yong-hui, ZENG Shu-hua, YANG Min-feng, GUO Shi-ping, LIU Lei. Determination of the concentration of flue-cured tobacco volatile oils by UV-spectrophotography[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(18): 116-120.

References

[1] 于建军,宫长荣. 烟草原料初加工[M]. 北京:中国农业出版社, 2009.
[2] 中国烟叶生产购销公司.烤烟分级国家标准培训教材[M]. 北京:中国标准出版社,2005.
[3] 谢利丽,郭利,朱金峰,等. 烤烟烟叶油分研究进展[J]. 江西农业学报,2014,26(9):61-64.
[4] 詹金华. 烟叶化学及分析[M]. 昆明:云南科技出版社,1996.
[5] 杨君,高宏建,王宇,等. 超声辅助溶剂法提取烟草精油粗提物的研究[J].中国食品学报,2012,12(4):65-71.
[6] 张春涛,肖晓明,李海霞,等. GC-Ms/Ms指纹图谱应用于烟草的品质控制[J]. 食品工业,2016,37(7): 21-25.
[7] 吴鸣建,杨浩宇,李国富,等. 超声辅助索氏提取法提取烟草油工艺优化研究[J]. 香料香精化妆品,2016(3):11-17.
[8] 梁俊玉,杨强,马小梅,等. 甘肃三种杜鹃属植物挥发油含量及其抑菌活性研究[J]. 中国野生植物资源,2014,33(4): 9-10.
[9] 金文闻,熊悦,余龙江. 采用气相色谱技术对玛卡进行鉴别和质量评价[J]. 农业科学与技术(英文版),2006,7(3): 2-9.
[10] 崔於, 杨文娟. 川芎挥发油β-环糊精包合工艺影响因素的探讨[J]. 现代中药研究与实践,2013(4):40-42.
[11] 郑芳,李鹏,李志浩,等. 川芎挥发油脂质体制备工艺研究[J]. 中医药导报, 2009,15(12):69-71.
[12] 刘雷,王俊,雷强,等. 凉山和宜宾烤烟烟叶中特征挥发性物质的聚类分析与产地识别[J]. 烟草科技,2013(7):58-62.
[13] 张小全,杨志晓,李钦奎,等. 分光光度法定量测定烟草叶面分泌物含量研究[J]. 华南农业大学学报,2011,32(4):18-20.
[14] 魏福祥,韩菊,刘宝友. 仪器分析原理及技术[M]. 第2版. 北京:中国石化出版社, 2011.
[15] 张华,刘志广. 仪器分析简明教程[M]. 辽宁大连: 大连理工大学出版社, 2007.
[16] 潘省初. 计量经济学中级教程[M]. 第2版. 北京: 清华大学出版社, 2013.
[17] 鲁静,付凌燕,王旭. 质量分析方法验证中检出限和定量限测定方法探讨[J]. 中国药品标准, 2012, 13(1): 33-35.
[18] MAJI A K,MAITY N,BANERJI P,et al.A validated RP-HPLC-UV method for quantitative determination of puerarin in pueraria tuberosa DC tuber extract[J]. Pharmaceutical methods,2012, 3(2):79-83.
[19] 禹珊, 郭强胜, 王会琳, 等. 定量核磁共振波谱法同时测定中药虎杖中白藜芦醇和虎杖苷的含量[J]. 分析化学, 2015(1): 69-74.
[20] ABEBE B.Spectrophotometric method for the determination of caffeic acid complexation and thermodynamic properties[J]. International journal of biophysics, 2012, 2(2): 12-17.
[21] BOLOTIN P A, BARANOVSKY S F.Evstigneev spectrophotometric investigation of the hetero association of Caffeine and thiazine dye in aqueous solution[J]. Spectrochimica acta part A, 2006(64): 693-697.
[22] JOSEPH J,ELUVATHINGAL D, JEMMIS. Red-, Blue-,or No-Shift in hydrogen bonds: A unified explanation[J]. Journal of American chemical society,2007,129(15): 4620-4632.
[23] 潘中达, 赵保丹, 周进. 水溶液中染料吸光度与浓度的非线性关系[J]. 实验室研究与探索, 2014, 33(2):22-26.
[24] BARANOVSKII S F, BOLOTIN P A.Association of riboflavin, caffeing and sodium salicylate in aqueous solution[J]. Journal of applied spectroscopy, 2007, 74(2): 211-218.
[25] 殷海青. 光度分析中工作曲线偏离朗伯—比尔定律的原因[J]. 青海师专学报, 2004, 24(5): 63-66.
[26] 陈静, 涂莉, 林学东, 等. 改进布洛芬片溶出度的测定方法[J]. 华西药学杂志, 2005, 20(1): 64-65.
[27] 高宏建, 张献忠, 钟建军, 等. 水蒸汽蒸馏法提取烟草精油的研究[J]. 食品工业科技, 2011, 31(10): 388-390.