Abstract: In order to determine the optimal conditions for the purification of Flammulina velutipes flavonoids by ADS-8 macroporous resin and the antibacterial and antioxidative properties of flavonoids after purification, the effects of pH and concentration of sample solution on the adsorption properties of flavonoids in resin and the effects of eluent dosage and eluent volume fraction on the analytical efficiency of flavonoids in the case of ethanol as eluent were studied, and the ability of purified flavonoids to inhibit Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella enteritidis was tested. The ability of scavenging 2,2-diazo-bis （3-ethyl-benzothiazole-6-sulfonic acid） diammonium salt （ABTS·） was detected. The results showed that the optimal conditions for this purification process were that the pH of the sample solution was 5, the concentration of the sample solution was 1.19 mg/mL, the volume fraction of the eluent ethanol was 70%, and the amount of ethanol was 140 mL. Under the above conditions, the purification ratio of flavonoids was 1.84 times. The flavonoids had inhibitory effects on four kinds of reference bacteria. The order of antibacterial ability was Escherichia coli> Salmonella enteritidis>Staphylococcus aureus>Bacillus subtilis. The results showed that the total flavonoids of Flammulina velutipes had the ability of scavenging ABTS·, indicating that it had antioxidant activity, but weaker than vitamin C.

Key words: ADS-8 macroporous resin, Flammulina velutipes flavonoids, purification, antibacterial, antioxidant