Research on the preparation and antibacterial activity of composite films of grape seed extract

doi:10.14088/j.cnki.issn0439-8114.2023.11.021

Abstract

Abstract: Chitosan and sodium alginate were used as film forming materials and glycerin as the plasticizer. The effects of chitosan, sodium alginate, glycerin concentration and grape seed extract on tensile strength and elongation at the break of composite films were investigated by the single factor test, and the orthogonal test was established on this basis. The antibacterial activity of the composite films of grape seed extract against Escherichia coli, Salmonella, Pseudomonas aeruginosa and Staphylococcus aureus was determined by the K-B method.The results showed that the best composite films forming process was sodium alginate concentration of 2.00%, chitosan concentration of 1.50%, glycerin concentration of 0.2%, and grape seed extract of 20%. The composite films had antibacterial activity against all four control bacteria, with the best effect against Escherichia coli, its antibacterial sphere diameter of 7.4 mm and the worst antibacterial effect against Pseudomonas aeruginosa. The test method was simple and rapid, and the composite films were stable, with certain mechanical properties, appropriate transparency and water absorption. Adding grape seed extract could increase the antibacterial properties of the composite films, which provided a new idea for the preparation of antibacterial composite films and provided reference for the preparation of film products in the food preservation industry.

Key words: grape seed extract, chitosan, sodium alginate, composite films, antibacterial

CLC Number:

TS206.4

GENG Zhi, FU Jia-le, GUO Wan-ting. Research on the preparation and antibacterial activity of composite films of grape seed extract[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(11): 118-124.

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