Diversity of arbuscular mycorrhizal fungi in the rhizosphere soil of Salvia miltiorrhiza at different cultivation years

doi:10.14088/j.cnki.issn0439-8114.2025.10.007

Abstract

Abstract: To investigate the role of arbuscular mycorrhizal fungi (AMF) in the continuous cropping obstacles of Salvia miltiorrhiza Bunge, a sequencing library was constructed using a two-round PCR amplification approach with AMF-specific primers, and the community structure and diversity of AMF in the rhizosphere soil of wild, two-year-old, and five-year-old S. miltiorrhiza plants were analyzed by high-throughput sequencing technology. The results showed that the root system of S. miltiorrhiza had obvious mycelium, vesicle and spore structures. The mycorrhizal infestation rate of two-year old and five-year old S. miltiorrhiza root systems was significantly higher than that of wild S. miltiorrhiza, and the spore density was significantly lower than that of wild S. miltiorrhiza. A total of 77 AMF species from 7 families and 7 genera were identified from all the samples, and Glomus was the absolutely dominant genus in the AMF community, accounting for 87.94% of the total. Redundancy analysis (RDA) and distance-based redundancy analysis (db-RDA) revealed significant differences in both α-diversity and community composition of AMF between the rhizosphere soil of two-year-old cultivated S. miltiorrhiza and that of wild or five-year-old cultivated plants. Alkali-hydrolyzable nitrogen and total nitrogen were identified as key factors influencing AMF community diversity and structure, respectively. Correlation analysis revealed that soil total nitrogen content was significantly positively correlated with the Shannon diversity index, the ACE index, and the number of OTUs. Alkali-hydrolyzable nitrogen content showed significant positive correlations with the ACE index and the number of OTUs. The relative abundance of Glomus was significantly positively correlated with the Pielou evenness index and the Shannon diversity index, but significantly negatively correlated with available phosphorus content. In conclusion, the micro-ecological environment of the five-year-old S. miltiorrhiza rhizosphere soil was similar to that of the wild sample. Furthermore, nitrogen was identified as the primary soil factor influencing AMF diversity and community structure, while available phosphorus was the key factor affecting the distribution of Glomus.

Key words: Salvia miltiorrhiza Bunge, cultivation years, rhizosphere soil, arbuscular mycorrhizal fungi (AMF), community diversity, community structure, continuous cropping

CLC Number:

S154.3

LI Li, PENG Dian, YANG Hao, LIU Rui-bo. Diversity of arbuscular mycorrhizal fungi in the rhizosphere soil of Salvia miltiorrhiza at different cultivation years[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 41-48.

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