Abstract: The differences of bacteria community structure among tobacco continuous cropping diseased soil, continuous cropping non-diseased soil and newly cultivated soil were compared, so as to provide a theoretical basis for reducing tobacco continuous cropping diseases through microbial means. Metagenome high-throughput sequencing technology was used to compare bacterial communities of three types of soils. The results showed that, the number of total and unique OTUs of bacteria in continuous cropping diseased soil was the highest, and the bacterial community was very similar and had high homology. Proportions of Cyanobacteria, Bacteroides, Pseudomonas, Arenimonas, Bellilinea, Arthrobacter, Flavobacterium and Nocardioids were significantly higher in continuous cropping diseased soil than those in continuous cropping non-diseased soil and newly cultivated soil. The bacterial community structure of continuous cropping non-diseased soil was quite different, and the homology was low. Bradyrhizobium, Aciditerrimonas, Mycobacterium, Sphingomonas, and Rhodoplanes had relatively high proportions, but the proportion of Nitrospira was low in newly cultivated soil, and the bacterial community structure had its own characteristics.

Key words: tobacco, continuous cropping tobacco-growing soil, diseased soil, metagenome, bacteria community