Establishment of quality grading standards for mother plants of Isatidis Radix for seed production

doi:10.14088/j.cnki.issn0439-8114.2026.04.027

Abstract

Abstract: Using the fresh weight of leaves, fresh weight of roots, main root length, rhizome diameter, middle root diameter, and number of leaves as candidate indicators, four key grading indices:Fresh root weight, rhizome diameter, middle root diameter, and main root length,were identified for Isatidis Radix mother plants used for seed production through correlation analysis, principal component analysis, and cluster analysis. Based on these findings, a three-grade classification standard for mother plants was established as follows: Grade Ⅰ: fresh root weight ≥ 189 g, rhizome diameter ≥ 2.5 cm, middle root diameter ≥ 2.0 cm, and main root length ≥ 30 cm; Grade Ⅱ: 189 g > fresh root weight ≥ 87 g, 2.5 cm > rhizome diameter ≥ 1.8 cm, 2.0 cm > middle root diameter ≥ 1.7 cm, and main root length ≥ 30 cm; Grade Ⅲ: 87 g > fresh root weight ≥ 58 g, 1.8 cm > rhizome diameter ≥ 1.4 cm, 1.7 cm > middle root diameter ≥ 1.4 cm, and main root length ≥ 30 cm. Quality analysis showed that the contents of (R,S)-goitrin and ethanol-soluble extracts in all graded samples met the standards specified in the Pharmacopoeia of the People’s Republic of China, with ethanol-soluble extract content being particularly prominent. According to this standard, superior individual plants were selected during the field harvesting process of Isatidis Radix production, and the selected elite plant population was used as mother plants for seed production.

Key words: Isatidis Radix, mother plants for seed production, grading standard, cluster analysis

CLC Number:

S567.09

LIU Fang-lin, WANG Xue-xue, WANG Li-li, REN Yong, ZHANG Hui-ye, LI Cheng-lian, CHEN Rong. Establishment of quality grading standards for mother plants of Isatidis Radix for seed production[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(4): 179-183.

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