Abstract: Using 35 pairs of SSR primers designed based on the strawberry genome sequence, 17 strawberry varieties were amplified and analyzed. The results showed that 33 pairs of primers were polymorphic, yielding 239 bands, of which 218 were polymorphic, with a polymorphic band rate of 91.2%. The number of polymorphic bands amplified by different primers ranged from 2 to 14, with an average of 15.15. The polymorphism information content (PIC) values at each locus ranged from 0.624 5 to 0.891 2, with an average of 0.798 8. The cluster analysis results revealed that the genetic distances among the 17 strawberry varieties ranged from 0.65 to 0.85. At a genetic distance threshold of 0.70, all tested materials could be classified into four distinct groups, containing 11, 2, 2, and 2 accessions, respectively. The construction of a fingerprint database of 17 strawberry varieties provided a technical basis for the identification of strawberry varieties, and the results provided a data basis for genetic breeding and variety improvement in the strawberry.

Key words: strawberry, SSR marker, cluster analysis, fingerprints, variety identification