HUBEI AGRICULTURAL SCIENCES ›› 2026, Vol. 65 ›› Issue (9): 40-47.doi: 10.14088/j.cnki.issn0439-8114.2026.09.008

• Resource & Environment • Previous Articles     Next Articles

Effects of combined application of organic fertilizer and microbial fertilizer on soil nutrients and enzyme activities in vineyards in southwest China

CHEN Peng1, LI Shuang-xiao1, SHI Liang-tao2, LU Ze-ping1, LI Hang1, CHEN Li-mei1, LI Xiao-ying1   

  1. 1. College of Soil and Water Conservation, Southwest Forestry University, Kunming 650224, China;
    2. Institute of Tropical Eco-Agriculture, Yunnan Academy of Agricultural Sciences, Yuanmou 651300, Yunnan, China
  • Received:2026-04-27 Online:2026-09-25 Published:2026-09-17

Abstract: This study aimed to clarify the comprehensive improvement effect of the combined application of organic fertilizer and microbial fertilizer on vineyard soil quality and to explore a scientific fertilization model for vineyards in Wuhua District, Kunming City, Yunnan Province. Two-year-old grapevines of the Shine Muscat variety were used as materials. Six treatments were set up, including a no-fertilization control (CK), high (G2, 135 g/plant) and low (G1, 90 g/plant) application rates of microbial fertilizer alone, high (B2, 30 g/plant) and low (B1, 20 g/plant) application rates of organic fertilizer alone, and their combined application (G1+B1, 90,20 g/plant). Main soil nutrient contents and enzyme activities were measured at the young fruit stage, the expansion stage and the ripening stage, and correlation analysis and principal component analysis were used to comprehensively evaluate each treatment. The results showed that all fertilization treatments increased soil nutrient contents and enzyme activities, and the combined application treatment was generally superior to the single application treatments. The combined application of organic fertilizer and microbial fertilizer effectively enhanced soil enzyme activities, accelerated nutrient cycling and availability processes, and thus improved the comprehensive soil fertility.Correlation analysis showed that there were mostly significant positive correlations between soil nutrients and enzyme activities, and organic matter and total nitrogen were the key factors driving the changes in enzyme activities. Three principal components were extracted by principal component analysis, with a cumulative variance contribution rate of 81.05%, and the comprehensive scores were ranked as G1+B1 > B2 > G2 > B1 > G1 > CK. The G1+B1 treatment had the best comprehensive performance and could be used as a recommended fertilization scheme for improving quality and efficiency in vineyards in southwest China.

Key words: organic fertilizer, microbial fertilizer, combined application, vineyard, soil nutrients, enzyme activity, southwest China

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