Isolation and identification of a cellulase-high-producing lactic acid bacterium strain

doi:10.14088/j.cnki.issn0439-8114.2025.09.025

Abstract

Abstract: The objective of this study was to enhance the utilisation of cellulose resources and reduce the production cost of microbial feed additives through the development of high-yielding cellulase strains. A total of 11 strains were isolated and cultured from the rumen contents of healthy dairy cows. The strains were identified by a combination of physiological and biochemical tests, PCR amplification and 16S rDNA sequencing. Congo red sodium carboxymethyl cellulose agar medium was selected for the initial screening of cellulase-producing bacterial strains, and the enzyme viability of the enzyme-producing strains was determined by the DNS method.Results showed that a high cellulase-producing strain of Lactobacillus paracasei was identified. In the context of optimally cultivated conditions, with 0.2% carboxymethyl cellulose sodium (CMC-Na) serving as the substrate, the cellulase activity attained 0.57 U/mL at 40 ℃ and pH 5.0.

Key words: cellulase, enzyme-producing bacteria, lactic acid bacterium, enzyme activity

CLC Number:

Q939.11⁺7

YING Yang-yan, LI Chun-ling, FENG Xiang-ren, SU Ya-quan, YU Zhu-guang, CHEN Zhong-wei, LONG Bo, HUANG Ping-sheng, LI Chang-ting, PENG Hao, LI Yu-hua, LIU Yu-jun. Isolation and identification of a cellulase-high-producing lactic acid bacterium strain[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 157-161.

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