Spectrometric investigation of the antioxidant activities of Terminalia polyphenol against DPPH free radicals

doi:10.14088/j.cnki.issn0439-8114.2019.08.028

Abstract

Abstract: The UV-Vis absorption spectrum of Terminalia polyphenol which is natural antioxidant and DPPH free radicals was investigated,and the determination wavelength and the time for the reaction of Terminalia polyphenol with DPPH free radicals were optimized. Furthermore,the DPPH free radical scavenging activity of Terminalia polyphenol was evaluated. In the optimized systems,the half inhibitory concentration （IC₅₀） of four Terminalia polyphenol against DPPH was ranked as follows： gallic acid（0.0009 2 mg/mL）﹤caffeic acid（0.003 43 mg/mL）<vanillic acid（0.55 mg/mL）<p-coumaric（2.27 mg/mL）, which were comparable with the standard antioxidant rutin, and the antioxidant activities of gallic acid and caffeic acid significantly better than rutin. The results suggest that gallic acid and caffeic are the main antioxidant active components in Terminalia.

Key words: Terminalia polyphenol, DPPH free radicals, ultraviolet-visible absorption spectrum, antioxidant activity

CLC Number:

LI Xiao-fen, XIONG Hua-bin, ZHANG Hai-fen, ZI Yuan-li, ZHANG Yan, GAO Yun-tao, ZHANG Yan-li. Spectrometric investigation of the antioxidant activities of Terminalia polyphenol against DPPH free radicals[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(8): 121-125.

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