湖北农业科学 ›› 2019, Vol. 58 ›› Issue (8): 121-125.doi: 10.14088/j.cnki.issn0439-8114.2019.08.028

• 检测分析 • 上一篇    下一篇

诃子多酚清除DPPH自由基的光谱学研究

李晓芬1, 熊华斌1, 张海芬1, 字园丽2, 张言1, 高云涛1, 张艳丽1   

  1. 1.云南民族大学化学与环境学院,昆明 650500;
    2.云南省大理州巍山县职业技术中学,云南 大理 672400
  • 收稿日期:2018-09-14 出版日期:2019-04-25 发布日期:2019-12-04
  • 通讯作者: 高云涛,教授,(电话)0872-65913041(电子信箱)2314972096@qq.com
  • 作者简介:李晓芬(1986-),女,云南大理人,讲师,硕士,主要从事民族药抗氧化研究,(电话)13577062851(电子信箱)wjplxf24@163.com
  • 基金资助:
    云南省应用基础研究计划项目(2017FD118); 国家自然科学基金项目(201367025)

Spectrometric investigation of the antioxidant activities of Terminalia polyphenol against DPPH free radicals

LI Xiao-fen1, XIONG Hua-bin1, ZHANG Hai-fen1, ZI Yuan-li2, ZHANG Yan1, GAO Yun-tao1, ZHANG Yan-li1   

  1. 1.School of Chemistry and Biotechnology,Yunnan Minzu University,Kunming,650500,China;
    2.Weishan Vocational Middle School,Dali 672400,Yunnan,China
  • Received:2018-09-14 Online:2019-04-25 Published:2019-12-04

摘要: 对天然抗氧化活性成分诃子多酚和DPPH自由基的紫外-可见吸收光谱特征进行了研究。确定了诃子多酚清除DPPH自由基反应体系的测定波长和反应时间。研究了诃子多酚清除DPPH自由基的活性,获得了诃子多酚清除DPPH自由基的半数抑制浓度IC50值为没食子酸(0.000 92 mg/mL)<咖啡酸(0.003 43 mg/mL)<香草酸(0.55 mg/mL)<对香豆酸(2.27 mg/mL),其中没食子酸和咖啡酸的活性明显优于芦丁。表明没食子酸和咖啡酸是诃子多酚中主要的抗氧化活性成分。

关键词: 诃子多酚, DPPH自由基, 紫外-可见吸收光谱, 抗氧化活性

Abstract: The UV-Vis absorption spectrum of Terminalia polyphenol which is natural antioxidant and DPPH free radicals was investigated,and the determination wavelength and the time for the reaction of Terminalia polyphenol with DPPH free radicals were optimized. Furthermore,the DPPH free radical scavenging activity of Terminalia polyphenol was evaluated. In the optimized systems,the half inhibitory concentration (IC50) of four Terminalia polyphenol against DPPH was ranked as follows: gallic acid(0.0009 2 mg/mL)﹤caffeic acid(0.003 43 mg/mL)<vanillic acid(0.55 mg/mL)<p-coumaric(2.27 mg/mL), which were comparable with the standard antioxidant rutin, and the antioxidant activities of gallic acid and caffeic acid significantly better than rutin. The results suggest that gallic acid and caffeic are the main antioxidant active components in Terminalia.

Key words: Terminalia polyphenol, DPPH free radicals, ultraviolet-visible absorption spectrum, antioxidant activity

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