HPLC紫外检测法同时分析水中甲萘威和阿特拉津

doi:10.14088/j.cnki.issn0439-8114.2019.15.026

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (15): 111-114.doi: 10.14088/j.cnki.issn0439-8114.2019.15.026

HPLC紫外检测法同时分析水中甲萘威和阿特拉津

赵文晋¹, 顾桂飞²

1.贵州省毕节市环境监测中心站, 贵州毕节 551700;
2.贵州省毕节市周驿茶场, 贵州毕节 551700

收稿日期:2019-02-21 发布日期:2019-11-13
作者简介:赵文晋（1988-）, 男, 河南唐河人, 工程师, 硕士, 主要从事环境化学与毒理研究, （电话）18386277630（电子信箱）zhaowenjin2007@163.com。
基金资助:
贵州省科技支撑计划项目（黔科合支撑[2016]2534号）; 黔科合重大专项（[2018]3013）; 贵州省科学技术基金计划项目（黔科合基础[2017]1083）

Simultaneous analysis of carbaryl and atrazine in water by HPLC with ultraviolet detection

ZHAO Wen-jin¹, GU Gui-fei²

1.Environmental Monitoring Station of Bijie, Bijie 551700, Guizhou, China;
2.Zhouyi Tea Plantation, Bijie 551700, Guizhou, China

Received:2019-02-21 Published:2019-11-13

摘要/Abstract

摘要： 建立了一种同时测定水中甲萘威和阿特拉津含量的高效液相色谱分析法。样品经乙酸乙酯萃取净化后, 使用XDB-C18色谱柱和紫外检测器, 以甲醇∶水=60∶40（V/V）为流动相, 流速1.0 mL/min, 柱温25 ℃, 进样量10.0 μL, 在221 nm波长下进行测定。结果表明, 在0.03~1.00 mg/L甲萘威和阿特拉津的峰面积与其质量浓度间均呈良好的线性关系, 相关系数均为0.999 9, 最低检出限分别为0.089 μg/L和0.184 μg/L。在添加水平为0.2~1.0 μg/L水样中甲萘威和阿特拉津的平均回收率分别为85.12%~100.14%和82.67%~105.51%, 相对标准偏差分别为1.60%~4.69%和2.36%~4.26%。该方法简单、快速、准确、灵敏, 具有良好的精密度和准确度, 适用于常规检测分析。

关键词: 甲萘威, 阿特拉津, 紫外检测, HPLC

Abstract: A method for simultaneous analysis of carbaryl and atrazine content in water was established by high performance liquid chromatography（HPLC） with ultraviolet detector. Samples were extracted and purified by ethyl acetate. The XDB-C18 column was used as chromatography column, and the mobile phase was a mixture of methanol-water（60∶40, V/V） at a flow rate of 1.0 mL/min. The column temperature kept 25 ℃, injection volume was 10.0 μL and the wavelength of detector was 221 nm. The results showed that within the range of 0.03~1.00 mg/L, it had good linear relationship between the peak area of carbaryl and atrazine and its mass concentration, and the linear correlation coefficient for carbaryl and atrazine was 0.999 9 and 0.999 9 respectively. The minimum detecion limits of quantitation of carbaryl and atrazine was 0.089 μg/L and 0.184 μg/L respectively. On addition level of 0.2~1.0 μg/L range, the recovery of carbaryl and atrazine for water was 85.12%~100.14% and 82.67%~105.51% respectively, and relative standard deviation（RSD） respectively was 1.60%~4.69% and 2.36%~4.26%. The method could be used for conventional analysis, which was simple, rapid, sensitive, accurate and with good precision.

Key words: carbaryl, atrazine, ultraviolet detection, high performance liquid chromatography

中图分类号:

赵文晋, 顾桂飞. HPLC紫外检测法同时分析水中甲萘威和阿特拉津[J]. 湖北农业科学, 2019, 58(15): 111-114.

ZHAO Wen-jin, GU Gui-fei. Simultaneous analysis of carbaryl and atrazine in water by HPLC with ultraviolet detection[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(15): 111-114.

参考文献

[1] SOLOMON K R, GIESY J P, LAPOINT T W, et al.Ecologican risk assessment of atrazine in north American surface waters[J].Environ Toxicol Chem, 2013, 32(1):10-11.
[2] STOKCR T E, LAWS S C, GUIDICI D L, et al.The effects of atrazine on puberty in male wistar rats:An evaluation in the protocol for assessment of pubertal development and thyroid function[J].Toxicological sciences, 2000, 58(1):50-59.
[3] MUNGER R, ISACSON P, HU S, et al.Intrauterine growth retardation in low a communities with herbicide-Contaminated drinking water supplies[J].Environmental health perspectives, 1997, 105(3):308-314.
[4] SUSANNE W A.Sensitive enzyme immunoassay for the detection of atrazine based upon sheep, antibodies[J].Analytical letters, 1992, 25(6):1317-1408.
[5] ZHENG T Z, ZAHM S H, CANTOR K, et al.Agricultural exposure to carbamate pesticides and risk of non-hodgkin lymphoma[J].Journal of occupational & environmental medicine, 2001, 43:641-649.
[6] 胡洁, 王以燕, 许建宁.农药致癌性的研究进展[J].农药, 2009, 48(10):708-717.
[7] GB/T5750.9-2006, 生活饮用水标准检验方法农药指标[S].
[8] HJ 578-2010, 氧化沟活性污泥法污水处理工程技术规范[S].
[9] 安永生, 赵林林.在线固相萃取-高效液相色谱法同时测定水中甲萘威和阿特拉津[J].分析仪器, 2017(4):42-46.
[10] 万巧玲, 王良超, 余薇薇.UPLC-MS-MS同时快速检测水样中5种有机污染物[J].环境科学与技术, 2018, 41(S1):288-292.
[11] 张明, 唐访良, 徐建芬, 等.超高效液相色谱-串联质谱法快速测定地表水中联苯胺、苦味酸、甲萘威、阿特拉津和溴氰菊酯[J].色谱, 2018, 36(9):866-872.
[12] 王绍臣, 张剑平.超高效液相色谱-串联四级杆质谱法快速测定阿特拉津、甲萘威、微囊藻毒素LR、丙烯酰胺和联苯胺[J].环境科学与管理, 2016, 41(10):111-113.
[13] 许百环. 固相萃取-高效液相色谱法同时测定水中甲萘威、阿特拉津和苯并[a]芘[J].福建分析测试, 2017, 26(1):14-18.
[14] 王超, 吕怡兵, 腾恩江, 等.超高压液相色谱荧光检测法快速测定水中痕量苯胺与联苯胺[J].分析测试学报, 2013, 32(1):32-37.
[15] GB3838-2002, 地表水环境质量标准[S].
[16] 樊德方. 农药残留量分析与检测[M].上海:上海科技出版社, 1982.116-139.

HPLC紫外检测法同时分析水中甲萘威和阿特拉津

Simultaneous analysis of carbaryl and atrazine in water by HPLC with ultraviolet detection

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

[1]	郭海姣, 刘进宝, 胡华, 王先军, 刘雯. HPLC法对壮瑶药狗仔花中绿原酸和咖啡酸含量的测定[J]. 湖北农业科学, 2020, 59(7): 188-190.
[2]	兰梅, 杨红丽, 胡靖锋, 张丽琴, 徐学忠, 和江明. HPLC法分析叶用芥菜种子中莱菔硫烷含量[J]. 湖北农业科学, 2020, 59(17): 154-157.
[3]	秦素红, 覃俏峰. HPLC法测定生大黄、清蒸大黄的大承气汤活性成分分析[J]. 湖北农业科学, 2020, 59(15): 129-135.
[4]	吴飞飞, 张笛, 周静, 王富荣, 何华平, 徐凌云. 三种桃果实中的儿茶素、表儿茶素含量比较[J]. 湖北农业科学, 2019, 58(9): 110-113.
[5]	陈向丹, 王峻, 胡深, 万珊, 董书香. 反相HPLC测定精制马拉硫磷溶液方法探讨[J]. 湖北农业科学, 2019, 58(21): 170-172.
[6]	陈静, 丁洁, 沙芮, 李瑞宾, 牛犇. 变换波长HPLC法同时测定药食同源降脂片中橙黄决明素、芦丁和槲皮素含量[J]. 湖北农业科学, 2019, 58(2): 111-114.
[7]	赵志新, 王通, 潘苗. 商洛桔梗茎叶中桔梗皂苷D动态研究[J]. 湖北农业科学, 2019, 58(17): 116-118.
[8]	张志, 吕建伟, 覃开羽, 吴敏, 张蓓, 李俊伟, 王莹, 冯秀芳, 卢森华. 基于HPLC结合化学计量学的银菊合剂指纹图谱研究[J]. 湖北农业科学, 2019, 58(16): 110-114.
[9]	肖秀丹, 黄友谊, 钟家成, 黄延政. HPLC对酸茶中儿茶素及游离氨基酸组分含量的测定[J]. 湖北农业科学, 2018, 57(9): 92-96.
[10]	艾丹, 陈秋生. HPLC-ICP-MS测定牛黄解毒片中砷形态分析的不确定度[J]. 湖北农业科学, 2018, 57(23): 132-135.
[11]	刘倩茹, 柏圣达, 赵国雨, 徐云峰, 杨最素. 北太平洋鱿鱼缠卵腺抗氧化酶解寡肽的制备[J]. 湖北农业科学, 2018, 57(13): 70-74.
[12]	张振东, 刘希望, 马宁, 申栋帅, 李剑勇. 乙酰氨基阿维菌素原位凝胶注射剂体外释放度测定方法研究[J]. 湖北农业科学, 2018, 57(10): 95-97.