湖北农业科学 ›› 2020, Vol. 59 ›› Issue (8): 161-163.doi: 10.14088/j.cnki.issn0439-8114.2020.08.037

• 生物工程 • 上一篇    下一篇

烟草多酚氧化酶生物信息学分析

张永芬, 王平   

  1. 曲靖市烟草公司陆良分公司,云南 曲靖 655000
  • 收稿日期:2019-08-10 出版日期:2020-04-25 发布日期:2020-07-03
  • 作者简介:张永芬(1977-),女,云南陆良人,助理农艺师,主要从事烟叶生产研究工作,(电话)13598087187(电子信箱)3619215306@qq.com。

Bioinformatics analysis of tobacco polyphenol oxidase

ZHANG Yong-fen, WANG Ping   

  1. Luliang Branch of Qujing Tobacco Company, Qujing 655000, Yunnan,China
  • Received:2019-08-10 Online:2020-04-25 Published:2020-07-03

摘要: 为对烟草多酚氧化酶进行生物信息学分析,利用ProtScale、PhyML和Meme工具读取烟草多酚氧化酶,进行疏水性、进化树和保守基序分析。结果表明,烟草多酚氧化酶各成员均为亲水性氨基酸;多酚氧化酶成员的进化树被划分为三类,其中烟草和番茄聚为一类,第二类中的成员最多;NtPPO1、NtPPO2和NtPPO3均含有相同的保守基序,NtPPO1和NtPPO2分布位置较为相似,而NtPPO3与其存在一定的差异。

关键词: 烟草, 多酚氧化酶, 生物信息

Abstract: In order to carry out bioinformatics analysis of polyphenol oxidase in tobacco, the tools of ProtScale, PhyML and Meme were used to read polyphenol oxidase, and the hydrophobicity, phylogenetic tree and conserved motif were analyzed. The results showed that all members of tobacco polyphenol oxidase were hydrophilic amino acids; the phylogenetic tree of polyphenol oxidase members was divided into three categories, among which tobacco and tomato were clustered, and the second group had the most members; NtPPO1, NtPPO2 and NtPPO3 contained the same conserved motifs, and the positions of NtPPO1 and NtPPO2 were similar, and there were some differences between NtPPO3 and them.

Key words: tobacco, polyphenol oxidase, biological information

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