关键词: 黑木耳, 多巴色素异构酶, 生物信息学分析

Abstract: Using the strain Mn150 of Auricularia auricula as test material,specific primer were designed to clone the dopachrome tautomerase gene. Bioinformatics analysis of the gene showed that the dopachrome tautomerase gene sequence length was 355 bp, encoding 112 amino acids, the molecular weight was 13.05 kDa, and the molecular formula was C₅₈₄H₉₂₁N₁₈₁O₁₅₃S₄, the isoelectric point was 10.35, which was a hydrophilic protein. The amino acid sequence of this protein contained a 4-Oxalocrotonate Tautomerase Superfamily conservative domain,no signal peptide, no transmembrane structure,made up of 20.54% α helix, 5.36% β ture, 54.46% random coil and 19.64% extended strand. These results can provide a foundation for further study on the function of dopachrome tautomerase gene in Auricularia auriculata.

Key words: Auricularia auricula, dopachrome tautomerase, bioinformatics analysis