湖北农业科学 ›› 2022, Vol. 61 ›› Issue (3): 161-164.doi: 10.14088/j.cnki.issn0439-8114.2022.03.033

• 生物工程 • 上一篇    下一篇

小飞鼠NADH脱氢酶亚基1基因(ND1)的生物信息学分析

廉明栋, 陈红, 杨孟平, 田新民   

  1. 牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011
  • 收稿日期:2021-09-02 出版日期:2022-02-10 发布日期:2022-03-11
  • 通讯作者: 田新民(1982-),男,黑龙江明水人,副教授,主要从事动物生态学研究,(电子信箱)swxtxm@126.com。
  • 作者简介:廉明栋(1995-),男,山东济宁人,硕士,研究方向为动物学,(电子信箱)374586634@qq.com
  • 基金资助:
    牡丹江师范学院研究生科技创新专项 (kjcx2020-61mdjnu; kjcx2020-14mdjnu); 黑龙江省自然基金项目(LH2020C071); 牡丹江师范学院科研项目(MQP201405)

Bioinformatics analysis of NADH dehydrogenase subunit 1 (ND1) gene in Pteromys volans

LIAN Ming-dong, CHEN Hong, YANG Meng-ping, TIAN Xin-min   

  1. College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011,Heilongjiang,China
  • Received:2021-09-02 Online:2022-02-10 Published:2022-03-11

摘要: NADH脱氢酶亚基1(ND1)是线粒体膜呼吸链NADH脱氢酶(复合物Ⅰ)的核心亚基,其作用是将电子从NADH转移到呼吸链,为研究小飞鼠(Pteromys volansND1基因的功能,利用生物信息学方法对ND1的理化性质、亲水/疏水性、二级结构、三级结构、跨膜结构、蛋白质修饰位点及同源性等进行了分析和预测。结果表明,ND1基因共编码318个氨基酸,是稳定亲水蛋白,蛋白质二级结构主要为α-螺旋和无规则卷曲,其次为延伸链;与其他18种动物的ND1氨基酸序列进行对比,发现小飞鼠与黑白飞鼠(Hylopetes alboniger)亲缘关系较近。

关键词: 小飞鼠(Pteromys volans), NADH脱氢酶亚基1, 呼吸链, 生物信息学

Abstract: NADH dehydrogenase subunit 1 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that functions in the transfer of electrons from NADH to the respiratory chain. To obtain the information of ND1 gene in Pteromys volans, bioinformatics methods were used in this article to analyze ND1 in there areas: physicochemical properties of ND1, hydrophilic/hydrophobic properties, secondary structure, tertiary structure, transmembrane structure, protein modification sites and homology of ND1 in Pteromys volans. The results in this research indicated that 318 amino acids were found in ND1 gene, which was a stable hydrophilic protein. The main secondary structure of ND1 protein was α-helix, random coil and extended strand. The siberian flying squirrel (Pteromys volans) and the particolored flying squirrel (Hylopetes alboniger) were found to be more closely related by compaired the ND1 gene in the siberian flying squirrel with 18 other animal species.

Key words: Pteromys volans, NADH dehydrogenase subunit 1, respiratory chain, bioinformatics

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