猪APOA2启动子与转录因子GATA1作用的研究

doi:10.14088/j.cnki.issn0439-8114.2020.22.032

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (22): 155-159.doi: 10.14088/j.cnki.issn0439-8114.2020.22.032

猪APOA2启动子与转录因子GATA1作用的研究

张凤¹, 李鑫², 陈昆³, 陈明新¹

1.湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064;
2.华中农业大学动物科技学院,武汉 430070;
3.武汉市农业农村局,武汉 430023

收稿日期:2020-07-06 出版日期:2020-11-25 发布日期:2020-12-30
通讯作者: 陈明新,研究员,研究方向为草食家畜遗传育种与繁育,(电子信箱)13807104106@163.com
作者简介:张凤(1988-),女,山东菏泽人,助理研究员,博士,研究方向为动物分子遗传育种,(电话)13260512579(电子信箱)zhangfeng0130@163.com

Study on the interaction of porcine APOA2 promoter and transcription factor GATA1

ZHANG Feng¹, LI Xin², CHEN Kun³, CHEN Ming-xin¹

1. Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Wuhan 430064,China;
2. College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;
3. Wuhan Municipal Agricultural and Rural Affairs Bureau,Wuhan 430023,China

Received:2020-07-06 Online:2020-11-25 Published:2020-12-30

摘要/Abstract

摘要： 对猪APOA2潜在启动子区进行克隆及活性分析,确定核心启动子区域,并进一步分析转录因子GATA1对APOA2基因转录活性的影响。结果表明,APOA2基因主要在猪的肝脏及脂肪组织中表达。双荧光素酶活性显示,猪APOA2基因5′端侧翼-813~-565 bp区域为核心启动子区,且-659~-565 bp区域内更可能存在一些促进APOA2转录的顺式作用元件。过表达GATA1基因结果显示,转录因子GATA1显著促进APOA2基因的转录;定点突变试验确认,转录因子GATA1可直接与APOA2基因启动子区的GATA1结合位点(-608~-599 bp)相结合。转录因子GATA1可直接靶向APOA2基因启动子区并促进APOA2的转录。

关键词: 猪, APOA2, GATA1, 启动子, 转录活性

Abstract: This study was aimed to clone the potential promoter region of porcine APOA2 gene and analyze its transcriptional activity,obtain its core promoter region,and analyze the effect of transcription factor GATA1 on the transcriptional activity of APOA2 gene for further studying the function of APOA2 gene in meat traits.The results showed that APOA2 gene was mainly expressed in liver and adipose tissue of white pigs.The results of dual luciferase activity indicated that there was the core promoter region of APOA2 gene5 'flanking region at -813 to -565 bp,and there were more likely to be some cis-acting elements promoting APOA2 transcription in the region of -659 to -565 bp.The results of GATA1 over-expression indicated that transcription factor GATA1 promoted the transcription of APOA2 gene.Site-directed mutagenesis assay confirmed that transcription factor GATA1 could directly bind to GATA1 binding sites (-608 to -599 bp) in the promoter region of APOA2 gene.Taken together,these results suggested that transcription factor GATA1 could directly target the promoter region of APOA2 gene and promoted APOA2 transcription.

Key words: porcine, APOA2, GATA1, promoter, transcriptional activity

中图分类号:

S828

张凤, 李鑫, 陈昆, 陈明新. 猪APOA2启动子与转录因子GATA1作用的研究[J]. 湖北农业科学, 2020, 59(22): 155-159.

ZHANG Feng, LI Xin, CHEN Kun, CHEN Ming-xin. Study on the interaction of porcine APOA2 promoter and transcription factor GATA1[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 155-159.

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猪APOA2启动子与转录因子GATA1作用的研究

Study on the interaction of porcine APOA2 promoter and transcription factor GATA1

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