PAM细胞中猪MMP14不同转录本检测及其生物信息学分析

doi:10.14088/j.cnki.issn0439-8114.2020.23.038

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (23): 169-173.doi: 10.14088/j.cnki.issn0439-8114.2020.23.038

PAM细胞中猪MMP14不同转录本检测及其生物信息学分析

吴俊静¹, 万绪玲², 黄藏宇¹, 乔木¹, 武华玉¹, 梅书棋¹, 彭先文¹

1.湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064;
2.江夏区动物卫生监督所,武汉 430200

收稿日期:2020-06-10 出版日期:2020-12-10 发布日期:2020-12-30
通讯作者: 彭先文(1974-),男,湖北恩施人,研究员,硕士,主要从事猪遗传育种研究,(电子信箱)pxwpal@163.com
作者简介:吴俊静(1984-),女,湖北武汉人,副研究员,博士,主要从事猪遗传育种研究,(电话)027-87389516(电子信箱)jeanne1106@126.com
基金资助:
湖北省重大科技创新项目(2019ABA084);湖北省创新群体项目(2018CFA014);湖北省农业科技创新中心创新团队项目(2019-620-000-001-18);湖北省创新体系项目(2018skjcx05)

Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis

WU Jun-jing¹, WAN Xu-ling², HUANG Cang-yu¹, QIAO Mu¹, WU Hua-yu¹, MEI Shu-qi¹, PENG Xian-wen¹

1. Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Wuhan 430064,China;
2. Animal Health Supervision Institute of Jiangxia District,Wuhan 430200,China

Received:2020-06-10 Online:2020-12-10 Published:2020-12-30

摘要/Abstract

摘要： 基质金属蛋白酶14(MMP14)是基质金属蛋白酶家族中首个被发现的膜型基质金属蛋白酶。MMP14蛋白能水解多种细胞外基质组分和血清蛋白,促进细胞外基质降解,并可水解细胞因子、趋化因子和生长因子等。研究发现猪肺泡巨噬细胞(PAM)中存在2种猪MMP14转录本,利用荧光定量PCR技术检测了PRRSV感染的猪PAM细胞中猪MMP14基因不同转录本的表达水平变化,发现PRRSV感染后导致转录本NM_214239.2显著下调表达(P<0.05),而转录本XM_021098303.1极显著上调表达(P<0.01)。通过生物信息学分析发现,2种转录本编码的蛋白前肽区序列存在明显差异。XM_021098303.1编码的蛋白比NM_214239.2编码的蛋白序缺少了RRKR基序和PG_binding_1功能区,可能导致MMP14蛋白不具有功能活性。推测PRRSV感染后抑制MMP14活性,与PRRSV刺激细胞因子与趋化因子的大量表达相关。然而,MMP14在PRRSV感染中的具体作用还有待进一步研究。

关键词: 猪, MMP14, 不同转录本, PRRSV

Abstract: Matrix metalloproteinases 14 (MMP14) is the first discovered membrane-type matrix metalloproteinases in the matrix metalloproteinases super family.MMP14 protein can hydrolyze various extracellular matrix components and serum proteins,promote extracellular matrix degradation,and hydrolyze cytokines,chemokines and growth factors.In this study,two different porcine MMP14 transcripts were found in pig alveolar macrophages (PAM).The expression levels of different transcripts of MMP14 gene in porcine PAM cells infected with PRRSV were detected by fluorescence quantitative PCR,the results showed that the expression level of transcript NM_214239.2 significantly reduced (P<0.05),while XM_021098303.1 significantly incresed (P<0.01) in PRRSV infected PAMs.Bioinformatics analysis results showed that these two different transcripts encoded two different proteins.Compared with NM_214239.2 encoded protein,the RRKR motif and PG_binding_1 functional region were missing in XM_021098303.1 encoded protein,which might not havefunctionalactivity in the inactivation of MMP14 protein.It is speculated that the inactivation of MMP14 after PRRSV infection maybe relate to the high expression of cytokines and chemokines stimulated by PRRSV.However,the specific role of MMP14 in PRRSV infection still needs further study.

Key words: pig, MMP14, different transcripts, PRRSV

中图分类号:

S828
S813

吴俊静, 万绪玲, 黄藏宇, 乔木, 武华玉, 梅书棋, 彭先文. PAM细胞中猪MMP14不同转录本检测及其生物信息学分析[J]. 湖北农业科学, 2020, 59(23): 169-173.

WU Jun-jing, WAN Xu-ling, HUANG Cang-yu, QIAO Mu, WU Hua-yu, MEI Shu-qi, PENG Xian-wen. Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 169-173.

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PAM细胞中猪MMP14不同转录本检测及其生物信息学分析

Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis

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