滨海白首乌对高脂诱导肥胖小鼠的降脂减肥作用

doi:10.14088/j.cnki.issn0439-8114.2021.02.026

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (2): 126-129.doi: 10.14088/j.cnki.issn0439-8114.2021.02.026

滨海白首乌对高脂诱导肥胖小鼠的降脂减肥作用

陈其燕¹, 石云², 张言周¹, 洪键¹

1.盐城师范学院海洋与生物工程学院,江苏盐城 224007;
2.江苏医药职业学院药学院,江苏盐城 224002

收稿日期:2020-03-27 出版日期:2021-01-25 发布日期:2021-02-07
通讯作者: 洪键（1981-）,男（土家族）,高级实验师,博士,从事动物生理调控研究,（电话）0515-88258281（电子信箱）jian_hong602@sina.com。
作者简介:陈其燕（1995-）,女,贵州贵阳人,在读本科生,专业方向为生物工程,（电话）0515-88258281（电子信箱）26188636@qq.com。
基金资助:
国家级大学生创新创业训练计划项目（201710324006Z）; 江苏省高职院校青年教师企业实践项目（2018QYSJ041）; 江苏省产学研合作项目（FZ20180965）; 江苏省盐土生物资源研究重点实验室开放课题（72071666002G）

The alleviation efficacy of Binhai Cynanchum bungei on high-fat-induced obesity in mice

CHEN Qi-yan¹, SHI Yun², ZHANG Yan-zhou¹, HONG Jian¹

1. School of Marine and Biological Engineering, Yancheng Teachers’University, Yancheng 224007, Jiangsu, China;
2. Jiangsu Vocational College of Medicine, Yancheng 224002, Jiangsu, China

Received:2020-03-27 Online:2021-01-25 Published:2021-02-07

摘要/Abstract

摘要： 研究滨海白首乌对高脂诱导的小鼠肥胖的作用功效。试验选择3周龄SPF 雄性小鼠30只,随机分为对照组（Con）、高脂组（HF）和高脂加白首乌组（HBS）。连续饲喂9周,GTT检测葡萄糖耐受情况,检测小鼠体重和器官重等基础指标,测定血液中葡萄糖和甘油三酯的含量,HE染色观察脂肪组织和肌肉组织细胞数目及大小,油红染色观察肝脏组织脂质含量。结果表明,与HF组相比,HBS组小鼠葡萄糖耐受显著缓解;体重、棕色脂肪重、附睾脂肪重、棕色脂肪指数、附睾脂肪指数均显著降低（P<0.05）;并且血液中甘油三酯含量和葡萄糖浓度显著降低（P<0.05）;染色结果观察到HBS组小鼠肝脏中脂质含量降低,脂肪细胞减小。

关键词: 白首乌, 肥胖, 缓解, 脂代谢

Abstract: The alleviation efficacy of Cynanchum bungei on high-fat-induced obesity mice was studied. Thirty male mice were randomly divided into three groups, fed control diet （Con）, high-fat diet （HF, 45% energy from fat） and high-fat diet containing with Cynanchum bungei （HBS）. After feeding for 9 weeks, glucose tolerance was tested, body weight and organ weight were measured, plasma concentration of glucose and triglyceride were measured using respective commercial assay kits, HE staining and oil red staining were used to observe the number and size of adipose and muscle cells and the lipid content in liver. The results showed that fed mice with Cynanchum bungei significantly alleviated glucose intolerance. The body weight, brown fat weight, epididymal fat weight, brown fat index and epididymal fat index were significantly （P<0.05） reduced in HBS group, also the concentration of glucose and triglyceride in plasma were significantly decreased （P<0.05）. The lipid content in liver and the size of adipocyte were decreased in HBS group.

Key words: Cynanchum bungei, obesity, alleviate, lipid metabolism

中图分类号:

TS201.4

陈其燕, 石云, 张言周, 洪键. 滨海白首乌对高脂诱导肥胖小鼠的降脂减肥作用[J]. 湖北农业科学, 2021, 60(2): 126-129.

CHEN Qi-yan, SHI Yun, ZHANG Yan-zhou, HONG Jian. The alleviation efficacy of Binhai Cynanchum bungei on high-fat-induced obesity in mice[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(2): 126-129.

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滨海白首乌对高脂诱导肥胖小鼠的降脂减肥作用

The alleviation efficacy of Binhai Cynanchum bungei on high-fat-induced obesity in mice

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