食源性肥胖小鼠IRX3基因5'UTR区甲基化率研究

doi:10.14088/j.cnki.issn0439-8114.2019.20.041

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (20): 170-173.doi: 10.14088/j.cnki.issn0439-8114.2019.20.041

食源性肥胖小鼠IRX3基因5'UTR区甲基化率研究

袁明铭, 周静, 侯开领

四川省中医药科学院,成都 610041

收稿日期:2018-10-15 出版日期:2019-10-25 发布日期:2019-11-19
通讯作者: 侯开领,助理研究员,（电话）028-85247897（电子信箱）953207472@qq.com。
作者简介:袁明铭（1987-）,女,四川成都人,助理研究员,硕士,主要从事病原分子生物学研究,（电话）028-85247897（电子信箱）295741864@qq.com;
基金资助:
四川省实验动物资源共享与服务平台（2017TJPT0060）; 四川省公益性科研院所基本科研业务专项（A-2017N-27）

Research on IRX3 5'UTR methylation rate in food-induced obese mice abdominal adipose

YUAN Ming-ming, ZHOU Jing, HOU Kai-ling

Sichuan Academy of Chinese Medicin Sciences,Chengdu 610041,China

Received:2018-10-15 Online:2019-10-25 Published:2019-11-19

摘要/Abstract

摘要： 为了研究食源性肥胖小鼠腹部脂肪组织中IRX3基因5'UTR区甲基化情况及IRX3基因mRNA表达情况,将雄性C57BL/6J小鼠进行食源性肥胖造模,采用RT-qPCR法检测IRX3 mRNA表达情况,BSP （Bisulfite sequencing PCR,BSP）法检测IRX3基因5'UTR区甲基化情况。结果表明,肥胖组小鼠腹部脂肪组织IRX3基因相对表达量极显著高于对照组（P<0.01）;小鼠腹部脂肪组织IRX3基因5'UTR区甲基化率差异不显著,但有2个位点（-128 bp和-116 bp处）和1个位点（-182 bp处）的甲基化率在所有肥胖小鼠中分别达到62.5%、62.5%和75.0%,极显著高于其他位点。小鼠发生食源性肥胖,IRX3基因mRNA表达上调;食源性肥胖小鼠腹部脂肪组织IRX3基因表达上调与其5'UTR区甲基化率无关;食源性肥胖小鼠腹部脂肪组织IRX3基因5'UTR区3个特异位点的甲基化,可能参与了肥胖的发生。

关键词: 食源性肥胖, IRX3基因, 相对表达量, 5'UTR, 甲基化

Abstract: To investigate the IRX3 gene expression levels and the 5'UTR methylation rate in food-induced obese mice abdominal adipose, male obese C57BL/6J mice model was induced by feeding high-fat diet, RT-qPCR was used to detect the IRX3 expression levels and BSP was used to detect the 5'UTR methylation status. Results showed that Obese mice model was established successfully. Expression levels in obese mice were higher than normal controls（P<0.01）. The 5'UTR methylation rate had no significant difference in the two groups of mice, but the methylation rate in 2 loci（-128 bp and -116 bp） and 1 locus （-182 bp） were 62.5%, 62.5% and 75.0% respectively, all higher than other loci significantly. The IRX3 mRNA expression is up-regulated in food-induced obese mice. The IRX3 mRNA expression levels has nothing to do with it’s 5'UTR methylation rate. The methylation in 3 specific loci may be associated with obesity.

Key words: food-induced obesity, IRX3 gene, relative expression, 5'UTR, methylation

中图分类号:

袁明铭, 周静, 侯开领. 食源性肥胖小鼠IRX3基因5'UTR区甲基化率研究[J]. 湖北农业科学, 2019, 58(20): 170-173.

YUAN Ming-ming, ZHOU Jing, HOU Kai-ling. Research on IRX3 5'UTR methylation rate in food-induced obese mice abdominal adipose[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(20): 170-173.

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食源性肥胖小鼠IRX3基因5'UTR区甲基化率研究

Research on IRX3 5'UTR methylation rate in food-induced obese mice abdominal adipose

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