湖北农业科学 ›› 2020, Vol. 59 ›› Issue (11): 131-133.doi: 10.14088/j.cnki.issn0439-8114.2020.11.027

• 生物工程 • 上一篇    下一篇

安康市花魔芋matK基因的克隆及分析

周晓帆, 李静, 王瑞, 李锐, 王瑞娴   

  1. 安康学院现代农业与生物科技学院, 陕西 安康 725000
  • 收稿日期:2019-08-09 出版日期:2020-06-10 发布日期:2020-08-06
  • 通讯作者: 王瑞娴(1986-), 女, 讲师, 主要从事植物遗传育种研究, (电话)15191567546(电子信箱)wrx@aku.edu.cn。
  • 作者简介:周晓帆(1997-), 男, 陕西宝鸡人, 在读本科生, 研究方向为农学, (电话)15229863673(电子信箱)1556722036@qq.com;
  • 基金资助:
    安康学院硕士培育专项(2016AYXNZX019); 陕西省大学生创新创业训练计划项目(2016sxjy010)

Cloning and sequences analysis of matK gene of Amorphophallus konjac from Ankang city

ZHOU Xiao-fan, LI Jing, WANG Rui, LI Rui, WANG Rui-xian   

  1. College of Modern Agriculture and Biotechnology, Ankang University, Ankang 725000, Shaanxi, China
  • Received:2019-08-09 Online:2020-06-10 Published:2020-08-06

摘要: 以安康市花魔芋(Amorphophallus konjac)为材料提取基因组, 对matK基因序列进行扩增, 扩增产物经凝胶电泳分析后送生物技术公司测序, 并对测序结果进行分析。结果表明, 安康市花魔芋matK基因全长1 551 bp, 预测编码516个氨基酸;系统进化树结果显示, 花魔芋与Amorphophallus maxwellii、田阳魔芋(Amorphophallus corrugatus)和西盟魔芋(Amorphophallus krausei)的亲缘关系最近。

关键词: 花魔芋(Amorphophallus konjac), matK基因, 序列分析, 安康市

Abstract: The genome of the Amorphophallus konjac from Ankang city was extracted and the matK gene sequence was amplified. After confirmed by gel electrophoresis, amplified product was sequenced and analyzed. Results showed that the matK gene of Amorphophallus konjac from Ankang city was 1 551 bp in length and predicted to encode 516 amino acids. The phylogenetic tree showed that the Amorphophallus konjac had the closest relationship with Amorphophallus maxwellii, Amorphophallus corrugatus and Amorphophallus krausei.

Key words: Amorphophallus konjac, matK gene, sequence analysis, Ankang city

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