黄芪多糖在小鼠肝脏脂肪变性中的作用

doi:10.14088/j.cnki.issn0439-8114.2021.08.025

摘要/Abstract

摘要： 研究黄芪多糖对饮食诱导的小鼠肝脏脂肪变性的预防作用与机制。采用高脂果糖饮食诱导小鼠肝脏脂肪变性模型,造模同时采用400 mg/kg黄芪多糖灌胃预防组小鼠,观察各组小鼠的肝脏组织病理学特征,检测肝功能、血脂与肠道通透性相关指标,免疫印迹分析肝脏TLR4和MyD88蛋白表达。结果表明,与模型组相比,黄芪多糖预防组小鼠肝脏脂肪变性程度减轻,血清ALT、AST、ALP、TG、TC和GLU水平显著降低,VDL水平显著升高,肠道通透性减小,肝脏TLR4和MyD88蛋白表达水平下降。黄芪多糖可预防小鼠肝脏脂肪变性的发生,其机制与改善肠道屏障,调控TLR4/MyD88信号通路有关。

关键词: 肝脏脂肪变性, 黄芪多糖, 预防作用, Toll样受体4

Abstract: To study the preventive effect and mechanism of astragalus polysaccharides on diet induced hepatic steatosis of mice. The ICR mice were treated with high fat diet and 30% fructose water to building hepatic steatosis model. The mice of prevention groups were intragastric administration of 400 mg/kg astragalus polysaccharides at the same time of building model. After eight weeks, the liver histopathology, liver function, blood lipid and intestinal permeability were detected, and the expression of TLR4 and MyD88 protein in liver was analyzed by western blotting. Results showed that compared with the model group, astragalus polysaccharides caused the preventive group mice that the liver steatosis was reduced, the level of ALT, AST, ALP, TG, TC, GLU of serum were significantly reduced, the level of VDL was significantly increased, the intestinal permeability were decreased, and the expression level of TLR4 and MyD88 protein in liver was decreased. Astragalus polysaccharides can prevent hepatic steatosis, and its mechanism is related to improving intestinal barrier and regulating TLR4/MyD88 signaling pathway.

Key words: hepatic steatosis, astragalus polysaccharides, preventive effect, TLR4

中图分类号:

R282

王春花, 孙雪芳. 黄芪多糖在小鼠肝脏脂肪变性中的作用[J]. 湖北农业科学, 2021, 60(8): 121-125.

WANG Chun-hua, SUN Xue-fang. The function of astragalus polysaccharides in hepatic steatosis of mice[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(8): 121-125.

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