关键词: Nlu-miRNA-8, 双荧光素酶报告基因, Ccdc124

Abstract: To identify the influence of Nlu-miRNA-8 on the expression of Ccdc124（coiled-coil domain-containing protein 124-like）, based on the construction of the psi-CHECK2 luciferase reporter gene vector. Firstly, the Ccdc124 gene fragment containing the Nlu-miRNA-8 binding sequence was designed and synthesized. The luciferase reporter gene vector was constructed and transfected into Drosophila S2 cells. The effect of Nlu-miRNA-8 on the expression of Ccdc124 was observed by the change of luciferase activity. The results showed that Nlu-miRNA-8 significantly inhibited the fluorescence expression of the Ccdc124 group, but it did not significantly inhibit the fluorescence expression of the blank control and NC group. In this study, we successfully constructed a psi-CHECK2 vector of the 3′-UTR segment of the Cdcc124 gene, and preliminarily confirmed that Nlu-miRNA-8 can regulate the Cdcc124 gene.

Key words: Nlu-miRNA-8, dual luciferase reporter gene, Ccdc124