湖北农业科学 ›› 2022, Vol. 61 ›› Issue (24): 53-55.doi: 10.14088/j.cnki.issn0439-8114.2022.24.012

• 资源·环境 • 上一篇    下一篇

牦牛源富硒鼠李糖乳杆菌的优化培养

孙建春1, 吴征王2, 邹雨婷1, 石斌3, 吴庆侠4, 董海龙4, 田发益4   

  1. 1.西藏自治区农畜产品质量安全检验检测中心,拉萨 850013;
    2.渠县动物疾病预防控制中心,四川 达州 635200;
    3.西藏自治区农牧科学院畜牧兽医研究所,拉萨 850004;
    4.西藏农牧学院,西藏 林芝 860000
  • 收稿日期:2022-03-25 出版日期:2022-12-25 发布日期:2023-01-18
  • 通讯作者: 田发益(1967-),男,甘肃庄浪人,教授,主要从事西藏动物生产和植物活性成分研究,(电子信箱)xztfy@163.com。
  • 作者简介:孙建春(1990-),男,云南大理人,兽医师,硕士,主要从事畜禽健康养殖研究,(电话)15348901006(电子信箱)1987616977@qq.com;共同第一作者,吴征王(1994-),男,重庆梁平人,助理兽医师,主要从事畜禽健康养殖研究,(电话)17784073401(电子信箱)2076729187@qq.com。
  • 基金资助:
    国家科技部科技支撑计划项目(2016YFC0502004)

Optimized culture of yak-derived selenium-rich Lactobacillus rhamnosus

SUN Jian-chun1, WU Zheng-wang2, ZOU Yu-ting1, SHI Bin3, WU Qing-xia4, DONG Hai-long4, TIAN Fa-yi4   

  1. 1. Quality and Safety Inspection and Testing Center for Agricultural and Animal Products of Tibet Autonomous Region, Lhasa 850013,China;
    2. Quxian County Animal Disease Prevention and Control Center, Dazhou 635200, Sichuan,China;
    3. Animal Husbandry and Veterinary Research Institute, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850004,China;
    4. Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Tibet,China
  • Received:2022-03-25 Online:2022-12-25 Published:2023-01-18

摘要: 为了生产出硒量较高的牦牛源鼠李糖乳杆菌,采用含不同浓度亚硒酸钠的液体培养基、不同培养温度、不同加硒时间设计培养方案。结果表明,最佳培养条件是培养温度38 ℃、亚硒酸钠浓度12 μg/mL、加硒时间第6 h,培养24 h,转化率为50.34%、富硒量高达5 658.256 μg/g。本研究为生产富硒乳酸菌产品提供了较好的依据。

关键词: 鼠李糖乳杆菌, 富硒, 牦牛源

Abstract: In order to produce Lactobacillus rhamnosus of yak origin with high selenium content, a culture protocol was designed using the liquid medium containing different concentrations of sodium selenite, different incubation temperatures and different selenium addition times. The results showed that the best culture conditions were the incubation temperature of 38 ℃, sodium selenite concentration of 12 μg/mL, selenium addition time of the 6th h, and 24 h incubation, the conversion rate was 50.34% and the selenium enrichment was up to 5 658.256 μg/g. This study provided a better basis for the production of selenium-enriched lactic acid bacteria products.

Key words: Lactobacillus rhamnosus, selenium enrichment, yak source

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