关键词: 超高效液相色谱-四级杆飞行时间质谱, 三七, 真菌毒素

Abstract: An ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry screening and confirmation method was established for 9 types of mycotoxins in Panax notoginseng. The residual levels of 9 types of mycotoxins （Aflatoxin B₁、Aflatoxin B₂、Aflatoxin G₁、Aflatoxin G₂、Fumonitoxin B₁、Fumonitoxin B₂、Deoxynivalenol、Zearalenone、Ochratoxin A） in Panax notoginseng were quickly screened. The chromatographic column was Phenomenex Kinetex C18 （100 mm × 2.1 mm, 2.6 μm）, and gradient elution was performed using 0.1% formic acid aqueous solution and 0.1% formic acid methanol solution as mobile phases. Positive ion scanning and the matrix matching external standard method were used for quantification. The results showed that 9 types of mycotoxins had good linear relationships within the concentration ranges of 0.1~100.0, 5.0~1 000.0, and 0.5~500.0 μg/L, respectively,the correlation coefficients were all greater than 0.990 00, and the quantitative limit of the method was 0.3~15.0 μg/kg. At three spiked levels of high, medium, and low concentrations, the recovery rate was 71.22%~98.57%, and the relative standard deviation was 2.31%~6.72%. This method could match the precise mass numbers and fragment ion information of primary and secondary mass spectra in the mass spectrometry database without reference materials, reducing experimental costs. It had the advantages of simplicity, rapidness, efficiency, and accuracy, and was suitable for the rapid screening and determination of mycotoxins residues in Panax notoginseng.

Key words: ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry, Panax notoginseng, mycotoxins