关键词: 地方杏, 种质资源, 荧光SSR标记, 遗传多样性, 湖北省

Abstract: To explore the genetic diversity of local apricot variety resources in Hubei Province, 24 local apricot varieties materials were taken as test materials, and SSR molecular markers combined with fluorescence capillary electrophoresis technology were used for the study. The results showed that 14 pairs of primers from apricots, peaches, almonds, and cherries amplified a total of 162 polymorphic effective allele loci, with an average of 11.571 alleles per locus. The total number of effective alleles was 90.312, with an average of 6.451 effective alleles per locus. The average values of observed heterozygosity （Ho）, expected heterozygosity （He）, and Shannon's information index （I） were 0.652, 0.813, and 2.031, respectively. The average value of polymorphic information content （PIC） was 0.794, indicating that the primers for the selected related species had high universality and rich polymorphism. At a similarity coefficient of 0.580, 24 varieties were divided into three large groups. The first group was independently composed of Hongping apricot, the second group consisted of four varieties including Langmei and Xingmei et al, and the third group consisted of 19 varieties including rice apricot and Wuzu apricot. It could be seen that local apricot varieties in Hubei Province had rich genetic diversity, and SSR specific primers could effectively identify Hubei local apricot varieties.

Key words: local apricot, germplasm resources, fluorescent labeled SSR marker, genetic diversity, Hubei Province