棉花黄萎病高效拮抗细菌的筛选及其防治效果

doi:10.14088/j.cnki.issn0439-8114.2024.12.012

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (12): 67-72.doi: 10.14088/j.cnki.issn0439-8114.2024.12.012

棉花黄萎病高效拮抗细菌的筛选及其防治效果

张德政¹, 聂太礼¹, 杨军², 柯兴盛¹, 闫得朋¹, 聂樟清³, 钟敏¹

1.江西省经济作物研究所,南昌 330000;
2.江西省江棉科技开发有限公司,江西九江 332000;
3.江西省农业技术推广中心,南昌 330000

收稿日期:2023-11-21 出版日期:2024-12-25 发布日期:2025-01-08
通讯作者: 钟敏（1982-）,男,副研究员,博士,（电话）15170290585（电子信箱）327071001@qq.com。
作者简介:张德政（1992-）,男,河南太康人,助理研究员,硕士,主要从事土壤微生态研究,（电话）15690606791（电子信箱）zhangdezheng2016@163.com;聂樟清（1975-）,江西丰城人,高级农艺师,（电话）13870620741（电子信箱）nzq.nc@163.com。
基金资助:
江西省棉花产业技术体系资金项目（JXRS-22）

Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt

ZHANG De-zheng¹, NIE Tai-li¹, YANG Jun², KE Xing-sheng¹, YAN De-peng¹, NIE Zhang-qing³, ZHONG Min¹

1. Cash Crops Research Institute of Jiangxi Province, Nanchang 330000, China;
2. Jiangxi Jiangmian Technology Development Co.,Ltd., Jiujiang 332000,Jiangxi, China;
3. Jiangxi Agricultural Technology Promotion Center, Nanchang 330000, China

Received:2023-11-21 Published:2024-12-25 Online:2025-01-08

摘要/Abstract

摘要： 以棉花黄萎病菌（Verticillium dahliae Kleb）为指示菌,采用对峙培养、代谢物抑菌试验筛选高活性生防菌株。通过盆栽试验明确供试菌株的防病效果。结果表明,从棉花根际土壤分离的1 000余株细菌中筛选到162株具有生防效果的菌株,采用平板对峙法进行复筛,最终获得4株综合效果较好的菌株。其中菌株XJ5-6和XJ6-96的抑菌效果较好,抑菌带宽度均超过5.0 mm,抑菌率分别达67.54%和66.86%。盆栽试验结果表明,菌株XJ5-6和XJ6-96混合接种对棉花黄萎病具有较好的防治作用,其防治效果在28 d和40 d分别为32.2%和29.9%,菌株XJ6-96能提高棉花种子的萌发率。

关键词: 棉花黄萎病, 根际细菌, 高效拮抗细菌, 抑菌活性

Abstract: Using Verticillium dahliae Kleb as indicator bacteria, the biocontrol strains with high activity were screened by confronation culture and metabolite inhibition test. The preventive effect of the tested strain was determined by pot experiment. The results showed that 162 strains of bacteria with biocontrol effect were screened from more than 1 000 strains isolated from cotton rhizosphere soil, and 4 strains with good comprehensive effect were obtained by using the plate face-off method. Strains XJ5-6 and XJ6-96 showed better inhibitory effects, with the band width exceeding 5.0 mm and the inhibitory rate reaching 67.54% and 66.86%, respectively. The results of pot experiment showed that XJ5-6 and XJ6-96 combined inoculation had a good control effect on verticillium wilt of cotton, and the control effect was 32.2% and 29.9% on 28 and 40 days, respectively. XJ6-96 could improve the germination rate of cotton seed.

Key words: cotton verticillium wilt, rhizosphere bacteria, highly effective antagonistic bacteria, antagonistic activity

中图分类号:

张德政, 聂太礼, 杨军, 柯兴盛, 闫得朋, 聂樟清, 钟敏. 棉花黄萎病高效拮抗细菌的筛选及其防治效果[J]. 湖北农业科学, 2024, 63(12): 67-72.

ZHANG De-zheng, NIE Tai-li, YANG Jun, KE Xing-sheng, YAN De-peng, NIE Zhang-qing, ZHONG Min. Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(12): 67-72.

参考文献

[1] KLOSTERMAN S J,ATALLAH Z K,VALLAD G E,et al.Diversity, pathogenicity, and management of Verticillium species[J]. Annual review of phytopathology,2009,47(1):39-62.
[2] 尹梦亭. 阿克苏棉区土壤微生物多样性分析及棉花黄萎病生防菌的筛选[D]. 新疆石河子:石河子大学,2008.
[3] 陈明,穆凯热姆·阿卜来提,刘政,等.响应面法优化链霉菌LG-9发酵条件及对棉花黄萎病菌的抑菌作用[J]. 湖北农业科学,2018,57(8):71-76.
[4] 张德政,朱荷琴,冯自力,等.“入田”拌种对棉花枯黄萎病的防治效果[J]. 中国棉花,2020,47(2):30-33.
[5] WELLER D M,RAAIJMAKERS J M,GARDENER B B M,et al. Microbial populations responsible for specific soil suppressiveness to plant pathogens[J]. Annual review of phytopathology,2002,40(1):309-348.
[6] RYBAKOVA D,CERNAVA T,KÖBERL M,et al. Endophytes-assisted biocontrol: Novel insights in ecology and the mode of action of Paenibacillus[J]. Plant and soil,2016,405(1/2):125-140.
[7] HAN Q,WU F L,WANG X N,et al.The bacterial lipopeptide iturins induce Verticillium dahliae cell death by affecting fungal signalling pathways and mediate plant defence responses involved in pathogen-associated molecular pattern-triggered immunity[J]. Environmental microbiology,2015,17(4):1166-1188.
[8] 周京龙,冯自力,冯鸿杰,等.棉花内生蜡状芽孢杆菌YUPP-10对棉花黄萎病的防治作用及机制[J]. 中国农业科学,2017,50(14):2717-2727.
[9] 刘元元,庞学兵,李国,等.棉花黄萎病生防菌的筛选及挥发性抑菌物质检测[J]. 西北农业学报,2019,28(5): 820-829.
[10] 赵丽红,冯自力,李志芳,等.棉花抗黄萎病鉴定与评价标准的商榷[J]. 棉花学报,2017,29(1):50-58.
[11] PIERSON E A,WELLER D M.Use of mixtures of fluorescent pseudomonads to suppress take off and improve the growth of wheat[J]. Phytopathology,1994,84(9):940-947.
[12] BERENDSEN R L,VISMANS G,YU K,et al.Disease-induced assemblage of a plant-beneficial bacterial consortium[J]. The ISME journal,2018,12(6):1496-1507.
[13] SANTHANAM R,LUU V T,WEINHOLD A,et al.Native root-associated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping[J]. Proceedings of the national academy of sciences of the United States of America,2015,112(36):E5013-E5020.
[14] YACHI S,LOREAU M.Biodiversity and ecosystem productivity in a fluctuating environment: The insurance hypothesis[J]. Proceedings of the national academy of sciences,1999,96(4):1463-1468.
[15] WEI F,SHANG W J,YANG J R,et al.Spatial pattern of Verticillium dahliae microsclerotia and cotton plants with wilt symptoms in commercial plantations[J]. Plos one,2015,10(7):e132812.
[16] MALLON C A,ELSAS J D A,SALLES J F. Microbial Invasions: The process, patterns, and mechanisms[J]. Trends in microbiology,2015,23(11):719-729.
[17] ZHANG C S,KONG F Y.Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants[J]. Applied soil ecology,2014,82:18-25.
[18] PARK M R,KIM Y C,LEE S,et al.Identification of an ISR-related metabolite produced by rhizobacterium Klebsiella oxytoca C1036 active against soft-rot disease pathogen in tobacco[J]. Pest management science,2009, 65(10):1114-1117.

棉花黄萎病高效拮抗细菌的筛选及其防治效果

Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt

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