碳源及不同浓度水杨酸对多根紫萍生理特性及淀粉积累的调控作用

doi:10.14088/j.cnki.issn0439-8114.2025.09.022

摘要/Abstract

摘要： 为探讨碳源及水杨酸(SA)对多根紫萍(Spirodela polyrrhiza)生理特性及淀粉积累的调控作用,在有碳源、无碳源条件下,施加不同浓度SA处理,测定相关生理生化指标。结果表明,随着SA浓度的升高,多根紫萍干重和鲜重均降低;而碳源存在时可缓解该抑制生长的效应。通过叶绿素荧光动力学分析发现,单位反应中心耗散的能量(DIo/RC)上升,而用于电子传递的能量(ETo/RC)、光合机构性能指数(PIabs)及综合性能参数(PItotal)下降;碳源存在可减缓上述变化幅度,从而减轻SA对光合效率的抑制作用。过氧化氢酶(CAT)活性仅在有碳源时随SA浓度呈上升的趋势;过氧化物酶(POD)活性呈上升的趋势,且在无碳源时增幅更大;超氧化物歧化酶(SOD)活性仅在有碳源时呈一定上升趋势,且在200 μmol/L SA时达到峰值。有碳源时丙二醛(MDA)含量呈先升后降的趋势,在200 μmol/L SA降到最低;无碳源时MDA持续累积。淀粉含量则随SA浓度升高而增加,且在有碳源时积累更多。

关键词: 碳源, 水杨酸, 多根紫萍(Spirodela polyrrhiza), 生理特性, 淀粉积累

Abstract: The regulatory effects of a carbon source and salicylic acid (SA) on the physiological characteristics and starch accumulation in Spirodela polyrrhiza were investigated. Under conditions with or without a carbon source,Spirodela polyrrhiza was treated with different concentrations of SA, and related physiological and biochemical indexes were determined. The results showed that both the dry and fresh weights of Spirodela polyrrhiza decreased with increasing SA concentration. However, the presence of a carbon source alleviated this growth inhibition. Chlorophyll fluorescence kinetics analysis indicated that the dissipated energy flux per reaction center (DIo/RC) increased, while the electron transport flux per reaction center (ETo/RC), the photosynthetic performance index (PIabs), and the comprehensive performance index (PItotal) decreased. The presence of a carbon source mitigated these changes, thereby reducing the inhibitory effect of SA on photosynthetic efficiency. Catalase (CAT) activity showed an upward trend with the increase of SA concentration only in the presence of a carbon source. Peroxidase (POD) activity showed an increasing trend, with a more pronounced increase in the absence of a carbon source. Superoxide dismutase (SOD) activity increased only in the presence of a carbon source, peaking at 200 μmol/L SA. In the presence of a carbon source, the malondialdehyde (MDA) content first increased and then decreased, reaching its lowest level at 200 μmol/L SA. Under carbon source starvation, the MDA content accumulated continuously. Starch content increased with increasing SA concentration and was higher in the presence of a carbon source.

Key words: carbon source, salicylic acid, Spirodela polyrrhiza, physiological characteristics, starch accumulation

中图分类号:

Q945.79

黄婷, 贾榕, 向蓓蓓. 碳源及不同浓度水杨酸对多根紫萍生理特性及淀粉积累的调控作用[J]. 湖北农业科学, 2025, 64(9): 134-140.

HUANG Ting, JIA Rong, XIANG Bei-bei. Regulatory effects of carbon source and different concentrations of salicylic acid on the physiological characteristics and starch accumulation in Spirodela polyrrhiza[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 134-140.

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