三种药剂对斜纹夜蛾幼虫的毒力作用及解毒酶活性的影响

doi:10.14088/j.cnki.issn0439-8114.2025.11.012

摘要/Abstract

摘要： 研究斜纹夜蛾[Spodoptera litura (Fabricius)]幼虫在甲维盐·虫螨腈(EBC)、斜纹夜蛾核型多角体病毒(Spodoptera litura nuclear polyhedrosis virus, SpltNPV)和苏云金杆菌(Bacillus thuringiensis, BT)3种药剂毒力胁迫下的死亡率与解毒酶活性。采用浸虫法研究EBC、SpltNPV和BT对斜纹夜蛾4龄幼虫的毒力作用,同时采用酶标仪测定上述3种药剂对羧酸酯酶(Carboxylesterase,CarE)、多功能氧化酶(Multifunctional oxidase,MFO)和谷胱甘肽S-转移酶(Glutathione S-transferase,GSTs)活性的影响。结果表明,采用EBC、SpltNPV和BT处理斜纹夜蛾4龄幼虫,12 h后幼虫出现行动迟缓,体色变深,体壁褶皱,体长和体宽缩短。EBC稀释2 500~7 500倍时,斜纹夜蛾4龄幼虫的校正死亡率为60.71%~100.00%,致死中时间为76.70~111.50 h。SpltNPV稀释375~1 500倍时,校正死亡率为67.86%~100.00%,致死中时间为74.17~116.60 h。而BT稀释750~3 000倍时,校正死亡率为78.57%~100.00%,致死中时间为83.00~120.00 h。采用EBC稀释5 000倍、SpltNPV稀释750倍和BT稀释1 500倍分别处理斜纹夜蛾4龄幼虫,12~48 h内各处理幼虫体内CarE活性增加,MFO活性降低,GSTs活性则呈波动性变化。

关键词: 斜纹夜蛾[Spodoptera litura (Fabricius)], 药剂, 毒力, 解毒酶, 活性

Abstract: An investigation was conducted on the mortality and detoxification enzyme activities of Spodoptera litura (Fabricius) larvae under the toxic stress of three pesticides: Emamectin benzoate · chlorfenapyr (EBC), Spodoptera litura nucleopolyhedrovirus (SpltNPV), and Bacillus thuringiensis (BT). The immersion method was used to study the toxic effects of EBC, SpltNPV, and BT on the 4th instar larvae of Spodoptera litura. Meanwhile, a microplate reader was employed to determine the effects of these three insecticides on the activities of carboxylesterase (CarE), multifunctional oxidase (MFO), and glutathione S-transferase (GSTs). The results showed that after 12 h of treatment with EBC, SpltNPV, and BT, the 4th instar larvae of Spodoptera litura displayed symptoms including sluggish movement, darkened body color, wrinkled body walls, and a reduction in both body length and width. When EBC was diluted within the range of 2 500 to 7 500 times, the corrected mortality rate of the 4th instar larvae of Spodoptera litura ranged from 60.71% to 100.00%, with the median lethal time (LT₅₀) spanning from 76.70 to 111.50 h. When SpltNPV was diluted between 375 and 1 500 times, the corrected mortality rate ranged from 67.86% to 100.00%, and the LT₅₀ was between 74.17 and 116.6 h. When BT was diluted from 750 to 3 000 times, the corrected mortality rate ranged from 78.57% to 100.00%, and the LT₅₀ ranged from 83.00 to 120.00 h. When the 4th instar larvae of Spodoptera litura were treated with EBC diluted 5 000 times, SpltNPV diluted 750 times, and BT diluted 1 500 times, an increase in CarE activity and a decrease in MFO activity were observed in each treatment group within the range between 12 h and 48 h, whereas GSTs activity exhibited fluctuating changes.

Key words: Spodoptera litura (Fabricius), pesticide, toxicity, detoxification enzyme, activity

中图分类号:

张文梅, 路天, 李小珍, 李小军, 张友武, 王利兵, 程谦, 张志高. 三种药剂对斜纹夜蛾幼虫的毒力作用及解毒酶活性的影响[J]. 湖北农业科学, 2025, 64(11): 96-101.

ZHANG Wen-mei, LU Tian, LI Xiao-zhen, LI Xiao-jun, ZHANG You-wu, WANG Li-bing, CHENG Qian, ZHANG Zhi-gao. Toxicity and detoxification enzyme activity effects of three pesticides on Spodoptera litura larvae[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(11): 96-101.

参考文献

[1] 桑松,王政,齐江卫,等.斜纹夜蛾抗药性研究进展[J].环境昆虫学报,2013,35(6):808-814.
[2] 秦厚国,汪笃栋,丁建,等.斜纹夜蛾寄主植物名录[J].江西农业学报,2006,18(5):51-58.
[3] AHMAD M,ARIF M,AHMAD M.Occurrence of insecticide resistance in field populations of Spodoptera litura (Lepidoptera: Noctuidae) in Pakistan[J].Crop protection,2007,26(6):809-817.
[4] MALLIKARJUNA N,KRANTHI K R,JADHAV D R,et al.Influence of foliar chemical compounds on the development of Spodoptera litura (Fab.) in interspecific derivatives of groundnut[J].Journal of applied entomology,2004,128(5): 321-328.
[5] 刘世涛,王忠跃,刘永强,等.斜纹夜蛾在葡萄上的种群生命表[J].植物保护,2019,45(1):58-61,74.
[6] 周晓梅,黄炳球.斜纹夜蛾抗药性及其防治对策的研究进展[J].昆虫知识,2002,39(2):98-102.
[7] SAHU B,PACHORI R,NAVYA R N,et al.Extent of damage by Spodoptera litura on cabbage[J].Journal of entomology and zoology studies,2020,8(3):1153-1156.
[8] 饶荣莲. 烟草斜纹夜蛾的发生特点及综合防治措施[J].农业科技通讯,2023(9):200-202.
[9] 贾世平,曾维爱,吴小森,等.斜纹夜蛾核型多角体病毒与赤眼蜂联用对斜纹夜蛾的室内防治效果[J].植物保护,2022,48(6):307-312,361.
[10] 门友均,唐明丽,邓光宙,等.12%甲维·虫螨腈悬乳剂对柑橘潜叶蛾的防控效果[J].南方园艺,2021,32(5):36-38.
[11] 韦日健. 12%甲维·虫螨腈对草地贪夜蛾的防治效果试验[J].农业与技术,2020,40(7):31-32.
[12] MONTELLA I R,SCHAMA R,VALLE D.The classification of esterases: An important gene family involved in insecticide resistance—a review[J].Memorias do instituto oswaldo cruz,2012, 107(4):437-449.
[13] 任娜娜,谢苗,尤燕春,等.羧酸酯酶及其介导昆虫抗药性的研究进展[J].福建农林大学学报(自然科学版),2014,43(4):337-344.
[14] 张红英,赤国彤,张金林,等.昆虫解毒酶系与抗药性研究进展[J].河北农业大学学报,2002,25(S1):193-195.
[15] 葛天成,尹飞,胡琼波,等.MBF2转录调控小菜蛾谷胱甘肽S-转移酶代谢氯虫苯甲酰胺的功能[J].中国农业科学,2023, 56(4):665-673.
[16] 蒋兴川,沈怿丹,孙劲超,等.氯虫苯甲酰胺和甲维盐对草地贪夜蛾幼虫的毒力及解毒酶活性的影响[J].环境昆虫学报,2019,41(5):961-967.
[17] 陈凤菊,高希武,雷明庆,等.单宁酸对棉铃虫谷胱甘肽S-转移酶的影响[J].昆虫学报,2003,46(6):684-690.
[18] 李小珍,刘映红.食料对南亚果实蝇解毒酶活力的影响[J].昆虫学报,2007,50(10):989-995.
[19] 陈巨莲,倪汉祥,孙京瑞,等.小麦几种主要次生物质对麦长管蚜几种酶活力的影响[J].昆虫学报,2003,46(2):144-149.
[20] HAMMOND R B,PEDIGO L P,POSTON F L.Green cloverworm leaf consumption on greenhouse and field soybean leaves and development of a leaf-consumption Model12[J].Journal of economic entomology,1979,72(5):714-717.
[21] 龙莹,刘建萍,伍绍龙,等.不同品种烟草对斜纹夜蛾幼虫生长和营养利用效率的影响[J].中国烟草学报,2020,26(3):91-97.
[22] 汤心砚,谭琳,曾维爱,等.不同杀虫剂对烟草斜纹夜蛾的室内及田间防效[J].中国植保导刊,2018,38(5):58-60,71.
[23] 徐洁,王绍坤,马龙海,等.不同类型生物杀虫剂对烟草斜纹夜蛾室内药效试验[J].西南农业学报,2012,25(3):912-916.
[24] 王迎春,李兰英,龚雪蛟,等.6种杀虫剂对茶园斜纹夜蛾的防治效果[J].安徽农业科学,2019,47(15):145-147.
[25] 龙友华,赵红梅.12%虫螨腈·甲维盐悬浮剂对十字花科蔬菜主要害虫的生物活性及田间药效[J].植物保护,2009,35(4):164-167.
[26] 曾任森,苏贻娟,叶茂,等.植物的诱导抗性及生化机理[J].华南农业大学学报,2008,29(2):1-6.
[27] TIWARI S,PELZ-STELINSKI K,STELINSKI L L.Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides[J].Pest management science,2011,67(1): 94-99.
[28] PISKORSKI R,DORN S.How the oligophage codling moth Cydia pomonella survives on walnut despite its secondary metabolite juglone[J].Journal of insect physiology,2011,57(6):744-750.
[29] DESPRÉS L,DAVID J P,GALLET C. The evolutionary ecology of insect resistance to plant chemicals[J].Trends in ecology & evolution,2007,22(6):298-307.
[30] 王瑞龙,孙玉林,梁笑婷,等.6种植物次生物质对斜纹夜蛾解毒酶活性的影响[J].生态学报,2012,32(16):5191-5198.
[31] 贾变桃,焦鹏,杨素梅.虱螨脲亚致死浓度对小菜蛾保护酶系和解毒酶系活力的影响[J].植物保护学报,2016,43(2):293-299.
[32] 尹显慧,吴青君,李学锋,等.多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响[J].农药学学报,2008,10(1):28-34.
[33] 李浩,陈亚平,鲁智慧,等.草地贪夜蛾和斜纹夜蛾幼虫体内保护酶及解毒酶对2种杀虫剂的响应比较[J].南方农业学报,2021,52(3):559-569.
[34] 张文成,王开运,牛芳,等.虫螨腈胁迫对甜菜夜蛾保护酶系和解毒酶系的诱导效应[J].植物保护学报,2009,36(5):455-460.
[35] 梁炜博,向兴,王学贵,等.噻虫啉亚致死剂量对斜纹夜蛾解毒酶系活性与生长繁殖的影响[J].昆虫学报,2017,60(10):1187-1197.
[36] 安志兰,褚栋,郭笃发,等.寄主植物对B型烟粉虱(Bemisia tabaci)几种主要解毒酶活性的影响[J].生态学报,2008,28(4):1536-1543.