湖北农业科学 ›› 2026, Vol. 65 ›› Issue (1): 7-11.doi: 10.14088/j.cnki.issn0439-8114.2026.01.002

• 资源·环境 • 上一篇    下一篇

不同环境下水稻穗部性状的QTL检测及稳定性分析

孙晓璇, 陈楚楚, 王传之, 王小雷   

  1. 宿州学院生物与食品工程学院,安徽 宿州 234000
  • 收稿日期:2025-08-15 出版日期:2026-01-25 发布日期:2026-02-10
  • 通讯作者: 王小雷,男,讲师,博士,主要从事作物遗传育种及种质资源创制研究,(电子信箱)wxl0vip@163.com。
  • 作者简介:孙晓璇(2000-),女,安徽合肥人,在读本科生,专业方向为生物技术,(电子信箱)370862921@qq.com。
  • 基金资助:
    宿州学院博士科研启动基金项目(2021BSK049); 安徽省大学生创新创业训练计划项目(S202510379126); 安徽省宿州学院大学生创新创业训练计划项目(ZCXM24-209); 宿州学院“四新”研究与改革实践项目(szxy2024xnjy01); 宿州学院重点科研项目(2025yzd14)

QTL detection and stability analysis of rice panicle traits in different environments

SUN Xiao-xuan, CHEN Chu-chu, WANG Chuan-zhi, WANG Xiao-lei   

  1. School of Biological and Food Engineering, Suzhou University, Suzhou 234000, Anhui, China
  • Received:2025-08-15 Published:2026-01-25 Online:2026-02-10

摘要: 水稻(Oryza sativa L.)穗部性状遗传机制的解析及其优异等位基因的鉴定是作物遗传改良研究的重要方向,系统揭示控制穗部表型的数量性状位点(QTL)及其分子调控网络对水稻高产育种具有重要意义。利用昌恢121与Koshihikari构建的208个染色体片段置换系(CSSLs),分别在2种环境(2021年江西省南昌市和2022年海南省三亚市)下对穗长、一次枝梗数和二次枝梗数的QTLs进行定位。共定位到19个穗部性状QTLs,分别位于第1—6、8、11、12号染色体上,单个位点的表型贡献率为3.94%~10.51%。其中,qPL-8qPNB-2-2qSNB-12在2种环境下均能够被重复检测到。通过对4个染色体片段置换系(CSSL50、CSSL125、CSSL187和CSSL205)在qSNB-12位点的表型鉴定,结果表明该位点具有稳定的加性效应。

关键词: 水稻(Oryza sativa L.), 染色体片段置换系, 穗部性状, 数量性状位点(QTL), 稳定性, 不同环境

Abstract: The analysis of the genetic mechanism of panicle traits in rice (Oryza sativa L.) and the identification of its excellent alleles were important directions for crop genetic improvement research. It was of great significance to systematically reveal the quantitative trait loci (QTL) and its molecular regulatory network that controled the panicle phenotype for rice high-yield breeding. Using 208 chromosome segment substitution lines (CSSLs) constructed by Changhui 121 and Koshihikari, QTLs for panicle length, primary branch number and secondary branch number were mapped under two environments (Nanchang City, Jiangxi Province in 2021 and Sanya City, Hainan Province in 2022). A total of 19 QTLs for panicle traits were mapped on chromosomes 1-6,8,11 and 12, respectively. The phenotypic contribution rate of a single locus was 3.94%-10.51%. Among them, qPL-8, qPNB-2-2 and qSNB-12 could be repeatedly detected in both environments. The phenotypic identification of four CSSLs (CSSL50, CSSL125, CSSL187 and CSSL205) at qSNB-12 locus showed that this locus had a stable additive effect.

Key words: rice (Oryza sativa L.), chromosome segment substitution lines, panicle traits, quantitative trait locus(QTL), stability, different environments

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