小尾寒羊BMP4、Krt85、PPARGC1A基因多态性与肉质性状的关联分析

doi:10.14088/j.cnki.issn0439-8114.2026.02.024

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 162-166.doi: 10.14088/j.cnki.issn0439-8114.2026.02.024

小尾寒羊BMP4、Krt85、PPARGC1A基因多态性与肉质性状的关联分析

徐铁山¹, 杜秋明², 韩志松¹, 郝晓平³, 陈健², 白俊艳⁴

1.辽宁省沙地治理与利用研究所,辽宁阜新 123000;
2.延边朝鲜族自治州动物疫病预防控制中心,吉林延边 133000;
3.内蒙古自治区乌兰察布市卓资县农牧和科技局,内蒙古乌兰察布 012300;
4.河南科技大学动物科技学院,河南洛阳 471023

收稿日期:2025-11-01 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 白俊艳（1975-）,女,教授,博士,研究方向动物分子遗传育种,（电子信箱）junyanbai@163.com。
作者简介:徐铁山（1980-）,男,山东德州人,助理研究员,硕士,研究方向畜禽养殖,（电话）13624187565（电子信箱）xts8008@163.com。
基金资助:
国家自然科学基金项目（31201777）

Association analysis between BMP4, Krt85, and PPARGC1A gene polymorphism and meat quality traits in Small Tail Han sheep

XU Tie-shan¹, DU Qiu-ming², HAN Zhi-song¹, HAO Xiao-ping³, CHEN Jian², BAI Jun-yan⁴

1. Liaoning Institute of Sand Land Management and Utilization, Fuxin 123000, Liaoning, China;
2. Yanbian Chaoxianzu (Korean) Autonomous Prefecture Animal Disease Prevention and Control Center, Yanbian 133000, Jilin, China;
3. Agriculture, Animal Husbandry, and Science and Technology Bureau of Zhuozi County, Ulanqab City, Inner Mongolia Autonomous Region, Ulanqab 012300, Inner Mongolia , China;
4. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2025-11-01 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 利用InDel多态性分子标记方法,以小尾寒羊为研究对象,对BMP4、Krt85、PPARGC1A三个基因的四个多态位点与肉质性状（包括滴水损失、剪切力、水分、蒸煮损失、色泽、粗蛋白质、粗脂肪）进行关联分析,以期为提高小尾寒羊肉质品质提供科学依据。结果表明,①检测到三个基因的四个位点的多态性,并且四个多态位点均具有DD、ID、II基因型。②BMP4基因多态信息含量为0.371,在群体中处于中度多态性（0.25≤PIC<0.50）;Krt85-1基因位点的多态信息含量为0.337,在群体中处于中度多态性（0.25≤PIC<0.50）;Krt85-2基因位点的多态信息含量为0.375,在群体中处于中度多态性（0.25≤PIC<0.50）;PPARGC1A基因的多态信息含量为0.294,在群体中处于中度多态性（0.25≤PIC<0.50）。③在蒸煮损失方面,小尾寒羊BMP4基因的ID基因型显著低于DD基因型（P<0.05）。在剪切力方面,小尾寒羊PPARGC1A基因的DD基因型显著低于ID、II基因型（P<0.05）。在色泽方面,小尾寒羊Krt85-1基因位点的II基因型显著低于DD、ID基因型（P<0.05）。在粗脂肪方面,小尾寒羊Krt85-2基因位点的ID基因型显著低于II基因型（P<0.05）。BMP4、Krt85、PPARGC1A基因均可以作为提高小尾寒羊肉品质的候选基因。

关键词: 小尾寒羊, BMP4基因, Krt85基因, PPARGC1A基因, 多态性, 肉质性状

Abstract: InDel polymorphism was used to analyze the four polymorphic sites of the three candidate genes of BMP4, Krt85 and PPARGC1A genes (including drip loss, shear force, moisture, cooking loss, color, crude protein, and crude fat), so as to provide some scientific basis for improving the meat quality of the Small Tail Han sheep. The results showed that: ① polymorphisms in the four sites of the three genes were detected, and all four polymorphic sites had genotypes DD, ID and II. ② The polymorphic information content of the BMP4 gene was 0.371, and was moderate polymorphism in the population（0.25≤PIC<0.50）; the polymorphic information content of the Krt85-1 gene locus was 0.337, and was moderate polymorphism in the population（0.25≤PIC<0.50）; the polymorphic information content of the Krt85-2 gene locus was 0.375, and was moderate polymorphism in the population（0.25≤PIC<0.50）; The polymorphic information content of the PPARGC1A gene was 0.294, and was moderate polymorphism in the population（0.25≤PIC<0.50）. ③ In terms of cooking loss, the ID genotype of BMP4 gene was significantly lower than that of DD genotype (P<0.05). In terms of shear force, the DD genotype of PPARGC1A gene was significantly lower than that of ID and II (P<0.05). In terms of color, the II genotype of the Krt85-1 gene locus was significantly lower than the DD and ID genotypes (P<0.05). For crude fat, the ID genotype at the Krt85-2 locus was significantly lower than that of genotype II (P<0.05). In conclusion, the BMP4, Krt85, and PPARGC1A genes could all be used as candidate genes for improving the meat quality of Small Tail Han sheep.

Key words: Small Tail Han sheep, BMP4 gene, Krt85 gene, PPARGC1A gene, polymorphism, meat quality

中图分类号:

S826.8

徐铁山, 杜秋明, 韩志松, 郝晓平, 陈健, 白俊艳. 小尾寒羊BMP4、Krt85、PPARGC1A基因多态性与肉质性状的关联分析[J]. 湖北农业科学, 2026, 65(2): 162-166.

XU Tie-shan, DU Qiu-ming, HAN Zhi-song, HAO Xiao-ping, CHEN Jian, BAI Jun-yan. Association analysis between BMP4, Krt85, and PPARGC1A gene polymorphism and meat quality traits in Small Tail Han sheep[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 162-166.

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小尾寒羊BMP4、Krt85、PPARGC1A基因多态性与肉质性状的关联分析

Association analysis between BMP4, Krt85, and PPARGC1A gene polymorphism and meat quality traits in Small Tail Han sheep

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