花魔芋HSF基因家族全基因组鉴定与功能进化特征分析

doi:10.14088/j.cnki.issn0439-8114.2026.02.032

摘要/Abstract

摘要： 为深入解析花魔芋（Amorphophallus konjac）在高温胁迫下的分子响应机制,基于花魔芋全基因组数据,通过生物信息学方法系统鉴定热激转录因子（HSF）基因家族成员,并对其理化性质、进化关系、保守基序及蛋白互作网络进行分析。结果表明,在花魔芋基因组中共鉴定到28个花魔芋HSF（AkHSF）基因家族成员,不均匀分布于8条染色体上。通过构建系统进化树,将花魔芋与拟南芥（Arabidopsis thaliana）的HSF成员共同划分为5个亚群,揭示了基因家族的进化保守性。保守基序与基因结构分析表明,同一亚群成员具有相似的基序组成,暗示功能相近;而不同亚群间存在差异,提示功能发生分化。蛋白互作预测揭示了7个AkHSF成员间存在6种互作关系。AkHSF基因家族成员在热胁迫响应功能上既存在保守性,也发生了适应性进化。

关键词: 花魔芋（Amorphophallus konjac）, HSF基因家族, 全基因组, 生物信息学, 鉴定, 功能进化, 特征分析

Abstract: To elucidate the molecular response mechanism of Amorphophallus konjac under high-temperature stress, based on the whole-genome data of Amorphophallus konjac, the heat shock transcription factor (HSF) gene family members were systematically identified using bioinformatics methods, and their physicochemical properties, evolutionary relationships, conserved motifs, and protein interaction networks were analyzed. The results showed that a total of 28 AkHSF gene family members were identified in the Amorphophallus konjac genome, which were unevenly distributed on 8 chromosomes. By constructing a phylogenetic tree, the HSF members of Amorphophallus konjac and Arabidopsis thaliana were co-classified into five subgroups, revealing the evolutionary conservation of the gene family. The analysis of conserved motifs and gene structures indicated that members within the same subgroup shared similar motif compositions, suggesting functional similarity, while differences existed among different subgroups, implying functional divergence. Protein interaction prediction revealed six interaction relationships among seven AkHSF members. The AkHSF gene family members exhibited both conservation and adaptive evolution in their functions related to heat stress response.

Key words: Amorphophallus konjac, HSF gene family, whole-genome, bioinformatics, identification, functional evolution, characterization

中图分类号:

S632.3

蔡阳光, 厉健康, 段龙飞, 覃剑锋, 吴峻琪. 花魔芋HSF基因家族全基因组鉴定与功能进化特征分析[J]. 湖北农业科学, 2026, 65(2): 215-222.

CAI Yang-guang, LI Jian-kang, DUAN Long-fei, QIN Jian-feng, WU Jun-qi. Genome-wide identification and functional evolutionary characterization of the HSF gene family in Amorphophallus konjac[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 215-222.

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