湖北农业科学 ›› 2026, Vol. 65 ›› Issue (10): 150-154.doi: 10.14088/j.cnki.issn0439-8114.2026.10.022

• 药用植物 • 上一篇    下一篇

马蓝与桂越马蓝不同溶剂提取物的制备及体外抑菌活性比较

陈路1a, 刘钰1a, 黄丹娜1a, 潘宇1a, 李茵1a, 徐永莉1b, 肖璐2, 邓日建1a, 吴无畏1a   

  1. 1.广西壮族自治区药用植物园,a.西南濒危药材资源开发国家工程研究中心;b.广西道地药材高品质形成与应用重点实验室,南宁 530023;
    2.四川省畜牧科学研究院动物遗传育种四川省重点实验室,成都 610066
  • 收稿日期:2026-07-22 出版日期:2026-10-25 发布日期:2026-10-23
  • 通讯作者: 邓日建,男,副主任药师,主要从事中药、民族药的研究与开发,(电子信箱)893515688@qq.com。
  • 作者简介:陈 路(1979-),男,广西南宁人,副主任药师,硕士,主要从事中药及壮瑶药的研究与开发,(电子信箱)150388451@qq.com;吴无畏,男,研究员,主要从事中药及壮瑶药的研究与开发,(电子信箱)wuweiwu2013@163.com
  • 基金资助:
    四川省区域创新合作项目(2025YFHZ0307); 广西科技计划项目(桂科AD17292003)

Comparative study on the preparation and in vitro antibacterial activity of different solvent extracts from Baphicacanthus cusia and Strobilanthes bantonensis

CHEN Lu1a, LIU Yu1a, HUANG Dan-na1a, PAN Yu1a, LI Yin1a, XU Yong-li1b, XIAO Lu2, DENG Ri-jian1a, WU Wu-wei1a   

  1. 1a.National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development; 1b.Guangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;
    2. Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Sichuan Animal Science Academy, Chengdu 610066, China
  • Received:2026-07-22 Published:2026-10-25 Online:2026-10-23

摘要: 比较马蓝[Baphicacanthus cusia(Nees)Bremek.]和桂越马蓝(Strobilanthes bantonensis Lindau)不同极性提取物的体外抑菌活性,采用水提和有机溶剂提取法制备两种植物的不同溶剂提取物,通过琼脂平板打孔法测定各提取物对枯草芽孢杆菌(Bacillus subtilis)、金黄色葡萄球菌(Staphylococcus aureus)、绿脓杆菌(Pseudomonas aeruginosa)和大肠埃希菌(Escherichia coli)的体外抑制效果。结果表明,两种植物的水提取物、水提醇沉提取物和氯仿提取物对枯草芽孢杆菌、金黄色葡萄球菌、绿脓杆菌均有抑制作用,抑菌圈直径为5.41~16.99 mm,两者抑菌效果无显著差异;两种植物石油醚提取物对金黄色葡萄球菌、绿脓杆菌有抑制作用,抑菌圈直径为5.32~7.70 mm,两者抑菌效果无显著差异;两种植物丙酮提取物和不同浓度乙醇提取物对枯草芽孢杆菌、金黄色葡萄球菌有抑制作用,抑菌圈直径为5.94~11.45 mm,两者抑菌效果无显著差异;桂越马蓝70%乙醇和95%乙醇提取物对大肠埃希菌有抑制作用,抑菌圈直径分别为(6.42±0.54)mm和(7.74±0.55)mm,而马蓝相同提取物对大肠埃希菌无明显抑制作用。

关键词: 马蓝[Baphicacanthus cusia(Nees)Bremek.], 桂越马蓝(Strobilanthes bantonensis Lindau), 提取物, 抑菌活性, 替代药材

Abstract: To compare the in vitro antibacterial activities of extracts with different polarities from Baphicacanthus cusia (Nees) Bremek. and Strobilanthes bantonensis Lindau, aqueous extraction and organic solvent extraction methods were adopted to prepare extracts of the two plants using different solvents. The agar plate well diffusion method was applied to determine the in vitro bacteriostatic effects of all extracts against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The results showed that aqueous extracts, aqueous extracts obtained by water extraction and alcohol precipitation, and chloroform extracts from the two plants exhibited varying degrees of inhibitory activity against Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa, with inhibition zone diameters ranging from 5.41 to 16.99 mm, and no significant difference in antibacterial efficacy was observed between the two plant species. Petroleum ether extracts showed inhibitory effects on Staphylococcus aureus and Pseudomonas aeruginosa, with inhibition zone diameters of 5.32-7.70 mm, and their antibacterial activities also had no significant interspecific difference. Acetone extracts and ethanol extracts of different concentrations inhibited Bacillus subtilis and Staphylococcus aureus, with inhibition zone diameters of 5.94-11.45 mm, and no significant difference in performance between the two plants. Notably, the 70% ethanol and 95% ethanol extracts of Strobilanthes bantonensis Lindau showed inhibitory effects against Escherichia coli, with inhibition zone diameters of (6.42±0.54) mm and (7.74±0.55) mm, respectively, whereas the corresponding extracts of Baphicacanthus cusia (Nees) Bremek. showed no significant inhibitory effects against this bacterium.

Key words: Baphicacanthus cusia, Strobilanthes bantonensis, extracts, antibacterial activity, substitute medicinal material

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