响应面法优化糙米总黄酮提取工艺

doi:10.14088/j.cnki.issn0439-8114.2024.09.028

摘要/Abstract

摘要： 以胚芽糙米为材料,采用乙醇溶液提取总黄酮,先后开展单因素试验和响应面试验,考察提取温度、乙醇浓度、提取时间和液料比4个因素对糙米总黄酮提取率的影响。结果表明,乙醇提取糙米总黄酮的最佳条件为液料比20∶1、乙醇浓度80%、提取温度40 ℃和提取时间16 min,在此条件下,总黄酮得率为2.550%,与预测得率2.591%相比,相对误差仅为1.58%。优化后的方法比优化前更科学准确、操作简单、高效快捷和环保廉价,适用于大批量糙米总黄酮的提取。

关键词: 糙米, 黄酮, 乙醇提取法, 响应面法

Abstract: Using germ brown rice as the material, ethanol solution was used to extract total flavonoids, and single factor test and response surface test were carried out successively to investigate the influence of extraction temperature, ethanol concentration, extraction time and liquid material ratio on the extraction rate of total flavonoids from brown rice. The results showed that the best condition for ethanol extraction of total flavonoids from brown rice was the liquid material ratio of 20∶1, ethanol concentration of 80%, extraction temperature of 40 ℃ and extraction time of 16 min. Under this condition, the total flavonoids yield was 2.550%, and the relative error was only 1.58% compared with the predicted yield of 2.591%. The optimized method was more scientific, more accurate, simpler, more efficient,more environment-friendly and cheaper than before, and was suitable for large-scale extraction of total flavonoids from brown rice.

Key words: brown rice, flavonoids, ethanol extraction method, response surface methodology

中图分类号:

TQ914.1

李悦, 程建峰, 罗彤, 段依婧. 响应面法优化糙米总黄酮提取工艺[J]. 湖北农业科学, 2024, 63(9): 167-172.

LI Yue, CHENG Jian-feng, LUO Tong, DUAN Yi-jing. Optimization of the extraction of total flavonoids from brown rice by response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 167-172.

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