关键词: 啤酒, QuEChERS法, 高效液相色谱-串联质谱法, 氨基甲酸酯类农药, 残留

Abstract: To establish a QuEChERS high-performance liquid chromatography tandem mass spectrometry method for simultaneous determination of 10 carbamate pesticide residues in beer,beer samples were extracted with acetonitrile, and 1.5 g of sodium chloride was used for salting out. The purification agent was 30 mg PSA+15 mg GCB+885 mg anhydrous magnesium sulfate, mobile phase A was 0.01% formic acid aqueous solution+2 mmol/L ammonium formate solution, mobile phase B was methanol, and the chromatographic column was Shim pack GIST-HP C18-AQ （100 mm × 2.1 mm, 1.9 μm）. The analysis was carried out in multiple reaction monitoring mode （MRM） and the external standard method was used for quantification. The results showed that there was a good linear relationship among the 10 types of carbamate pesticides within the concentration range of 5~150 μg/L, and the correlation coefficients （R²） were all greater than 0.999 0,the LOD was 0.10-1.43 μg/L, the LOQ was 0.29-4.76 μg/L, the spiked recovery rate of the sample was 85.9%~118.0%, and the RSD was 1.4%~8.8% （n=6）. This method was accurate in quantification, simple in operation, and fast in analysis, and was suitable for rapid screening and quantitative analysis of various carbamate pesticide residues in beer.

Key words: beer, QuEChERS method, high performance liquid chromatography tandem mass spectrometry, carbamate pesticides, residues