不同樱桃番茄品种2-苯乙醇及其糖苷含量与相关基因的表达

doi:10.14088/j.cnki.issn0439-8114.2025.06.034

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (6): 207-214.doi: 10.14088/j.cnki.issn0439-8114.2025.06.034

不同樱桃番茄品种2-苯乙醇及其糖苷含量与相关基因的表达

史先鹏¹, 宋敏¹, 武丞宇¹, 刘立锋², 程斐¹, 马静¹

1.青岛农业大学园艺学院/山东省园艺作物基因改良工程实验室/青岛市园艺植物遗传改良与育种重点实验室,山东青岛 266109;
2.山东省农业科学院蔬菜研究所,济南 250100

收稿日期:2025-03-05 出版日期:2025-06-25 发布日期:2025-07-18
通讯作者: 马静（1990-）,女,山东日照人,副教授,博士,主要从事蔬菜遗传育种与分子生物学研究,（电话）19050505860（电子信箱）jma@qau.edu.cn。
作者简介:史先鹏（2000-）,男,山东枣庄人,在读硕士研究生,研究方向为番茄遗传育种与分子生物学,（电话）15621007330（电子信箱）20222102043@stu.qau.edu.cn;共同第一作者,宋敏(2000-),女,山东临沂人,在读硕士研究生,研究方向为番茄遗传育种与分子生物学,（电话）18763781065（电子信箱）20232107036@stu.qau.edu.cn
基金资助:
山东省自然科学基金资助项目（ZR2021QC018; ZR2024MC057）; 日喀则市科技人才引培行动暨科技计划“揭榜挂帅”项目（RKZ2023JB-02）

Content of 2-Phenylethanol and its glycosides and expression of associated genes in different cherry tomato cultivars

SHI Xian-peng¹, SONG Min¹, WU Cheng-yu¹, LIU Li-feng², CHENG Fei¹, MA Jing¹

1. College of Horticulture/Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province/Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants, Qingdao Agricultural University, Qingdao 266109, Shandong, China;
2. Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Received:2025-03-05 Published:2025-06-25 Online:2025-07-18

摘要/Abstract

摘要： 以4个樱桃番茄（Solanum lycopersicum L.）品种（千禧、小红、T7、釜山88）为试验材料,采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-QTOF-MS/MS）测定果实中2-苯乙醇、2-苯基乙基β-D-葡萄糖苷的含量。基于系统进化分析筛选潜在的糖基转移酶（UGT）基因,并利用实时荧光定量PCR（RT-qPCR）分析其表达模式,探究2-苯乙醇、2-苯基乙基β-D-葡萄糖苷的含量与相关基因表达水平之间的关联性。结果表明,不同番茄品种间2-苯乙醇、2-苯基乙基β-D-葡萄糖苷含量存在较大差异,均表现为千禧>小红>T7>釜山88,其中,千禧中2-苯乙醇、2-苯基乙基β-D-葡萄糖苷的含量均最高,分别为3 906.69、5 590.26 ng/g,均显著高于其他品种。通过系统发育分析,筛选出20个SlUGT基因,其中,SlUGT42、SlUGT54、SlUGT103、SlUGT104和SlUGT105在果实发育过程中表达量呈增加趋势。SlUGT103、SlUGT104、SlUGT105在千禧中表达水平最高,同时其2-苯乙醇及糖苷含量也达到最高水平;SlUGT103的相对表达量与2-苯乙醇、2-苯基乙基β-D-葡萄糖苷含量均呈显著正相关;SlUGT104的相对表达量与2-苯乙醇含量呈显著正相关。SlUGT103和SlUGT104是催化2-苯乙醇糖基化反应的关键葡萄糖基转移酶基因。

关键词: 樱桃番茄, 2-苯乙醇, 糖苷, 2-苯基乙基β-D-葡萄糖苷, 含量, 基因表达

Abstract: Four cherry tomato（Solanum lycopersicum L.） cultivars (Qianxi, Xiaohong, T7, and Fushan 88) were used as experimental materials. The contents of 2-phenylethanol and 2-phenylethyl β-D-glucoside in fruits were determined by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). Potential uridine diphosphate glycosyltransferase (UGT) genes were screened through phylogenetic analysis. Their expression patterns were analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR) to investigate the correlation between the contents of 2-phenylethanol/2-phenylethyl β-D-glucoside and related gene expression levels. The results showed significant differences in 2-phenylethanol and 2-phenylethyl β-D-glucoside contents among cultivars, with the order of Qianxi>Xiaohong>T7>Fushan 88. The highest contents of 2-phenylethanol (3 906.69 ng/g) and 2-phenylethyl β-D-glucoside (5 590.26 ng/g) were detected in Qianxi, both significantly higher than those in other cultivars.Phylogenetic analysis identified 20 SlUGT genes. Among them, SlUGT42, SlUGT54, SlUGT103, SlUGT104, and SlUGT105 showed increasing expression trends during fruit development.SlUGT103, SlUGT104, and SlUGT105 exhibited the highest expression levels in Qianxi, coinciding with its peak 2-phenylethanol and glycoside contents. The relative expression of SlUGT103 showed significant positive correlations with both 2-phenylethanol and 2-phenylethyl β-D-glucoside contents. The relative expression of SlUGT104 was significantly positively correlated with 2-phenylethanol content.SlUGT103 and SlUGT104 were identified as key glucosyltransferase genes catalyzing the glycosylation of 2-phenylethanol.

Key words: cherry tomato, 2-phenylethanol, glycoside, 2-phenylethyl β-d-glucoside, content, gene expression

中图分类号:

史先鹏, 宋敏, 武丞宇, 刘立锋, 程斐, 马静. 不同樱桃番茄品种2-苯乙醇及其糖苷含量与相关基因的表达[J]. 湖北农业科学, 2025, 64(6): 207-214.

SHI Xian-peng, SONG Min, WU Cheng-yu, LIU Li-feng, CHENG Fei, MA Jing. Content of 2-Phenylethanol and its glycosides and expression of associated genes in different cherry tomato cultivars[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 207-214.

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不同樱桃番茄品种2-苯乙醇及其糖苷含量与相关基因的表达

Content of 2-Phenylethanol and its glycosides and expression of associated genes in different cherry tomato cultivars

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