恩施州不同海拔五倍子没食子酸含量与生物活性评价

doi:10.14088/j.cnki.issn0439-8114.2026.01.019

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (1): 111-115.doi: 10.14088/j.cnki.issn0439-8114.2026.01.019

恩施州不同海拔五倍子没食子酸含量与生物活性评价

高翔^1,2, 郑润愽^1,2, 朱越^1,2, 李宇², 王晴芳², 罗凯¹, 何美军^1,2, 石爱华¹

1.恩施职业技术学院生物工程学院,湖北恩施 445000;
2.湖北省农业科学院中药材研究所/国家中药材产业技术体系恩施综合试验站,湖北恩施 445000

收稿日期:2024-10-21 出版日期:2026-01-25 发布日期:2026-02-10
通讯作者: 石爱华(1982-),女,副教授,博士,主要从事植物育种与栽培研究,(电子信箱)398348170@qq.com。
作者简介:高翔(1999-),男,河南三门峡人,在读硕士研究生,研究方向为天然产物研究与开发,(电子信箱)863385792@qq.com。
基金资助:
恩施州技术支撑科技计划项目(XYJ2023000044); 现代农业产业技术体系建设专项(CARS-21); 湖北省农业科学院青年科学基金项目(2024NKYJJ37)

Evaluation of gallic acid content and bioactivity in Galla chinensis at different altitudes in Enshi Prefecture

GAO Xiang^1,2, ZHENG Run-bo^1,2, ZHU Yue^1,2, LI Yu², WANG Qing-fang², LUO Kai¹, HE Mei-jun^1,2, SHI Ai-hua¹

1. College of Bioengineering, Enshi Polytechnic, Enshi 445000, China;
2. Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences/National Chinese Herbal Medicine Industry Technology System Enshi Comprehensive Experimental Station, Enshi 445000, Hubei, China

Received:2024-10-21 Published:2026-01-25 Online:2026-02-10

摘要/Abstract

摘要： 以恩施州海拔1 020 m(L-GC)与1 680 m(H-GC)采集的五倍子(Galla chinensis)为材料,采用HPLC法测定其没食子酸含量,并系统比较2种样品提取物的抑菌、抗癌及抗氧化活性。结果表明,H-GC的没食子酸含量(492.8 mg/g)显著高于L-GC(203.9 mg/g)。在抗氧化活性方面,H-GC提取物(H-MEGC)的DPPH清除IC₅₀为5.64 μg/mL,显著优于L-GC提取物(L-MEGC,IC₅₀为19.96 μg/mL)。2种提取物对A549肺癌细胞均具有显著抑制效果,IC₅₀分别为25.72 μg/mL(L-MEGC)与27.43 μg/mL(H-MEGC),二者组间无显著差异。在抑菌试验中,100 mg/mL的H-MEGC对部分供试菌株(枯草芽孢杆菌、苏云金芽孢杆菌、鲍曼不动杆菌)的抑制效果优于L-MEGC。随着海拔升高,恩施州五倍子的没食子酸含量增加,其抗氧化能力增强,对部分测试菌株的抑菌活性有所提高,而对A549细胞抑制活性无显著影响。

关键词: 五倍子(Galla chinensis), 恩施州, 海拔, 没食子酸, 抑菌活性, 抗氧化活性, 抗癌活性

Abstract: Galla chinensis samples collected from Enshi Prefecture at altitudes of 1 020 m (L-GC) and 1 680 m (H-GC) were utilized. The gallic acid content was determined by HPLC, and the antibacterial, anticancer, and antioxidant activities of the two extracts were systematically compared. The results showed that the gallic acid content of H-GC (492.8 mg/g) was significantly higher than that of L-GC (203.9 mg/g). In terms of antioxidant activity, the H-GC extract (H-MEGC) exhibited a DPPH scavenging IC₅₀ value of 5.64 μg/mL, which was significantly better than that of the L-GC extract (L-MEGC, IC₅₀=19.96 μg/mL). Both extracts significantly inhibited the proliferation of A549 lung cancer cells, with IC₅₀ values of 25.72 μg/mL (L-MEGC) and 27.43 μg/mL (H-MEGC), and there was no significant difference between the two groups. In antibacterial assays, H-MEGC at 100 mg/mL showed stronger inhibition against some tested strains (Bacillus subtilis, Bacillus thuringiensis, and Acinetobacter baumannii) compared to L-MEGC. With increasing altitude, the gallic acid content, antioxidant capacity, and antibacterial activity of Enshi Prefecture Galla chinensis against certain strains were enhanced, while its inhibitory effect on A549 cells was not significantly affected.

Key words: Galla chinensis, Enshi Prefecture, altitude, gallic acid, antibacterial activity, antioxidant activity, anticancer activity

中图分类号:

高翔, 郑润愽, 朱越, 李宇, 王晴芳, 罗凯, 何美军, 石爱华. 恩施州不同海拔五倍子没食子酸含量与生物活性评价[J]. 湖北农业科学, 2026, 65(1): 111-115.

GAO Xiang, ZHENG Run-bo, ZHU Yue, LI Yu, WANG Qing-fang, LUO Kai, HE Mei-jun, SHI Ai-hua. Evaluation of gallic acid content and bioactivity in Galla chinensis at different altitudes in Enshi Prefecture[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 111-115.

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恩施州不同海拔五倍子没食子酸含量与生物活性评价

Evaluation of gallic acid content and bioactivity in Galla chinensis at different altitudes in Enshi Prefecture

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