水稻类钙调蛋白基因家族鉴定及表达模式分析

doi:10.14088/j.cnki.issn0439-8114.2026.05.033

摘要/Abstract

摘要： 为系统鉴定水稻类钙调蛋白（OsCML）基因家族成员并解析其在生长发育与非生物胁迫响应中的潜在功能,利用生物信息学方法,基于全基因组水平共鉴定出44个OsCML基因,并对其理化性质、系统进化、基因结构、复制事件、共线性关系、顺式作用元件及表达模式进行综合分析。结果表明,44个OsCML基因不均匀分布于12条染色体上,大多数OsCML蛋白定位于细胞质和细胞膜;系统发育分析将OsCML蛋白划分为8个亚族;基因复制分析显示,片段复制是该家族扩张的主要驱动力;种间共线性分析揭示CML基因在单子叶与双子叶植物间具有高度保守的共线性关系;顺式作用元件分析表明,OsCML启动子区主要富集光响应、胁迫响应、激素响应及生长发育相关四大类元件。OsCML基因家族在7种组织中呈特异性与广泛表达差异,并在4种非生物胁迫下呈多样化响应,提示其在水稻抗逆适应中发挥关键作用。

关键词: 水稻, 类钙调蛋白, 基因家族, 鉴定, 表达模式

Abstract: To systematically identify the members of the rice calmodulin-like protein (OsCML) gene family and explore their potential functions in growth, development, and abiotic stress responses, bioinformatics methods were used to identify a total of 44 OsCML genes at the whole-genome level, and their physicochemical properties, phylogeny, gene structures, duplication events, syntenic relationships, cis-acting elements, and expression patterns were comprehensively analyzed. The results showed that the 44 OsCML genes were unevenly distributed on 12 chromosomes, and most OsCML proteins were localized in the cytoplasm and cell membrane.Phylogenetic analysis divided the OsCML proteins into eight subfamilies. Gene duplication analysis showed that segmental duplication was the main driving force for the expansion of this family. Interspecific synteny analysis revealed that CML genes had highly conserved syntenic relationships between monocot and dicot plants. Cis-acting element analysis indicated that the promoter regions of OsCML genes were mainly enriched with four types of elements related to light response, stress response, hormone response, and growth and development. The OsCML gene family showed specific and widespread expression differences in seven tissues, and exhibited diverse responses under four abiotic stresses, suggesting that they played key roles in the stress adaptation of rice.

Key words: rice, calmodulin-like protein, gene family, identification, expression pattern

中图分类号:

S511

杨蓝天, 余振渊, 谢艺. 水稻类钙调蛋白基因家族鉴定及表达模式分析[J]. 湖北农业科学, 2026, 65(5): 221-229.

YANG Lan-tian, YU Zhen-yuan, XIE Yi. Identification and expression pattern analysis of the calmodulin-like protein gene family in rice[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(5): 221-229.

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