利川市藤茶黄酮成分研究

doi:10.14088/j.cnki.issn0439-8114.2020.04.020

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (4): 98-101.doi: 10.14088/j.cnki.issn0439-8114.2020.04.020

利川市藤茶黄酮成分研究

李宇¹, 刘翠君¹, 艾伦强¹, 张宇¹, 卢新鹏², 何美军¹, 冉亚兰³

1.湖北省农业科学院中药材研究所,湖北恩施 445000;
2.湖北民族大学医学部,湖北恩施 445000;
3.恩施土家族苗族自治州农业农村局,湖北恩施 445000

收稿日期:2019-11-15 出版日期:2020-02-25 发布日期:2020-05-13
通讯作者: 何美军(1973-),男(土家族),湖北恩施人,副研究员,博士,主要从事中药材天然产物研究,(电子信箱)840940513@qq.com。
作者简介:李宇(1990-),男(土家族),湖北恩施人,助理研究员,硕士,主要从事中药材天然产物研究,(电话)15342857390(电子信箱)977956996@qq.com。
基金资助:
湖北省恩施州科技计划专项(XYJ2016000305)

Study on flavonoids from Ampelopsis grossedentata in Lichuan city

LI Yu¹, LIU Cui-jun¹, AI Lun-qiang¹, ZHANG Yu¹, LU Xin-peng², HE Mei-jun^1,2, RAN Ya-lan³

1.Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
2.Medical School,Hubei Minzu University,Enshi 445000,Hubei,China;
3.Agricultural and Rural Bureau of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,Hubei,China

Received:2019-11-15 Online:2020-02-25 Published:2020-05-13

摘要/Abstract

摘要： 从湖北省利川市齐岳山(海拔1 500~1 600 m)采集藤茶(Ampelopsis grossedentata)样品,采用60%乙醇超声提取总黄酮,利用紫外可见光分光光度计双波长(λ=208.4 nm,λ=293.2 nm)检测藤茶幼嫩茎尖总黄酮,平均含量高达453.67±11.26 mg/g;利用硅胶柱色谱及半制备高效液相分离到3个化合物,利用LC-HR-ESIMS、1D和2D NMR波谱数据进行分析,鉴定其结构分别为二氢杨梅素(1)、杨梅素(2)和花旗松素(3);通过高效液相(HPLC)检测分析发现,利川市藤茶样品中二氢杨梅素、杨梅素和花旗松素的含量为别186.31±10.43、13.83±1.32、14.76±2.26 mg/g。

关键词: 藤茶(Ampelopsis grossedentata), 总黄酮, 二氢杨梅素, 杨梅素, 花旗松素, 利川市

Abstract: The total flavonoids were extracted by 60% ethanol-ultrasound from the samples of Ampelopsis grossedentata collected from Qiyueshan mountain (1 500~1 600 m),Lichuan city, Hubei province. The total flavonoids in the young tips of Ampelopsis grossedentata was up to 453.67±11.26 mg/g detected by ultraviolet-visible spectrophotometer at the double-wavelength (λ=208.4 nm,λ=293.2 nm). Three compounds were purified from the organic extract using LC-HR-ESIMS,1D and 2D NMR spectral data analysis. Their structures were identified to be dihydromyricetin(1), myricetin(2),and taxifolin(3). The contents of dihydromyricetin, myricetin and taxifolin were 186.31±10.43, 13.83±1.32 and 14.76±2.26 mg/g respectively detected by HPLC.

Key words: Ampelopsis grossedentata, total flavonoids, dihydromyricetin, myricetin, taxifolin, Lichuan city

中图分类号:

S571.1

李宇, 刘翠君, 艾伦强, 张宇, 卢新鹏, 何美军, 冉亚兰. 利川市藤茶黄酮成分研究[J]. 湖北农业科学, 2020, 59(4): 98-101.

LI Yu, LIU Cui-jun, AI Lun-qiang, ZHANG Yu, LU Xin-peng, HE Mei-jun, RAN Ya-lan. Study on flavonoids from Ampelopsis grossedentata in Lichuan city[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(4): 98-101.

参考文献

[1] WANG T Y,LI Q,BI K S.Bioactive flavonoids in medicinal plants:Structure,activity and biological fate[J].Asian journal of pharmaceutical sciences,2018,13(1):12-23.
[2] WANG Y,CHEN S,YU O.Metabolic engineering of flavonoids in plants and microorganisms[J].Applied microbiology and biotechnology,2011,91(4):949.
[3] ZHANG X X,WU Q F,YAN Y L,et al.Inhibitory effects and related molecular mechanisms of total flavonoids in Mosla chinensis Maxim against H1N1 influenza virus[J].Inflammation research,2018,67(2):179-189.
[4] HWAN L,SEULKI L,YU S,et al.Anti-cancer effect of quercetin in xenograft models with EBV-associated human gastric carcinoma[J].Molecules,2016,21(10):1286.
[5] PATIL L,KALIWAL B B.Microalga Scenedesmus bajacalifornicus BBKLP-07, a new source of bioactive compounds with in vitro pharmacological applications[J].Bioprocess and biosystems engineering,2019,42(6):979-994.
[6] ZHANG X,XU Y,XUE H,et al.Antioxidant activity of vine tea (Ampelopsis grossedentata) extract on lipid and protein oxidation in cooked mixed pork patties during refrigerated storage[J].Food science & nutrition,2019,7(5):1735-1745.
[7] ZHENG Z W,GUO C X,MAO J,et al.Inhibitory effects of vine tea extract on proliferation of SGC-7901 human stomach cancer cells[J].Lishizhen medicine and materia medica research,2009,20(5):1158-1159.
[8] SHENG G,CHEN Y S,ABBASI A M,et al.Tannin fraction from Ampelopsis grossedentata leaves tea (Tengcha) as an antioxidant and α-lucosidase inhibitory nutraceutical[J].International journal of food science & technology,2016,51(12):2692-2700.
[9] XIE Y,LIU Q Q,LU Z Y,et al.The Processing technology of compound beverage and analysis on flavone of Ampelopsis grossedentata from fanjing mountain[J].Food research and development,2018,39(14):84-89.
[10] WANG L,LI X,ZHANG S,et al.Natural products as a gold mine for selective matrix metalloproteinases inhibitors[J].Bioorganic & medicinal chemistry,2012,20(13):4164-4171.
[11] WANG Y,ZHOU L,LI R,et al.Studies on the chemical constituents from Ampelopsis grossedentata[J].J Chin Med Mat,2002,25(4):254-256.
[12] JIA L G,WANG Y,SANG J C,et al.Dihydromyricetin inhibits alpha-synuclein aggregation,disrupts preformed fibrils,and protects neuronal cells in culture against amyloid-induced cytotoxicity[J].Journal of agricultural and food chemistry,2019, 76(14):3946-3955.
[13] PAN X X,CHEN T T,ZHANG Z J,et al.Activation of Nrf2/HO-1 signal with myricetin for attenuating ECM degradation in human chondrocytes and ameliorating the murine osteoarthritis[J].International immunopharmacology,2019,75(1):1-14.
[14] SUNIL C,XU B J,et al.An insight into the health-promoting effects of taxifolin(dihydroquercetin)[J].Phytochemistry,2019,7(5):1735-1745.

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利川市藤茶黄酮成分研究

Study on flavonoids from Ampelopsis grossedentata in Lichuan city

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