湖北农业科学 ›› 2021, Vol. 60 ›› Issue (11): 102-106.doi: 10.14088/j.cnki.issn0439-8114.2021.11.021

• 贮藏·加工 • 上一篇    下一篇

基于熵权法计算权重系数的正交试验优化益根调理膏醇提工艺

蒋林1,2, 郭勇秀1, 李芳婵1,2, 庞晓云3, 魏博伟1, 随家宁1   

  1. 1.广西中医药大学药学院,南宁 530001;
    2.广西壮瑶药工程技术研究中心,南宁 530001;
    3.广西中医药大学制药厂,南宁 530001
  • 收稿日期:2020-07-23 出版日期:2021-06-10 发布日期:2021-07-15
  • 作者简介:蒋 林(1965-),男,广西桂林人,教授级高级工程师,主要从事药品及保健食品的新产品研发,(电话)18677155109(电子信箱)1009100482@qq.com。
  • 基金资助:
    广西重点研发计划项目(桂科AB1850022); 南宁市重点研发计划项目(20193117); 广西创新驱动发展专项(桂科AA17204073); 广西高校中药提取纯化与质量分析重点实验室项目(桂教科研〔2014〕6号); 南宁市良庆区科学研究与技术开发计划项目(201910)

Optimization of extractions of Yigen Tiaoli paste by orthogonal design based on entropy weight method

JIANG Lin1,2, GUO Yong-xiu1, LI Fang-chan1,2, PANG Xiao-yun3, WEI Bo-wei1, SUI Jia-ning1   

  1. 1. School of Pharmacy, Guangxi University of Chinese Medicine,Nanning 530001,China;
    2. Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Nanning 530001,China;
    3. Pharmaceutical Factory,Guangxi University of Chinese Medicine,Nanning 530001,China
  • Received:2020-07-23 Online:2021-06-10 Published:2021-07-15

摘要: 以总黄酮、补骨脂素和圆柚酮提取量为评价指标,以乙醇体积分数、溶剂量、提取时间及提取次数为考察因素,优选益根调理膏醇提工艺。采用高效液相色谱法对补骨脂素和圆柚酮进行测定、紫外分光光度法对总黄酮进行测定。结果表明,总黄酮、补骨脂素和圆柚酮的权重系数分别为0.336 9 、0.335 1 、0.328 0,经优化后的醇提工艺为加70%乙醇12倍、提取2次、每次1.0 h。该提取工艺稳定可行,可作为益根调理膏醇提工艺。

关键词: 益根调理膏, 总黄酮, 补骨脂素, 圆柚酮, 醇提工艺

Abstract: The contents of total flavonoids, psoralen and nootkatone were used as the indicators, the alcohol concentration and volune, extraction time, number of repeated extractions were used as the influencing factors. orthogonal design was used to optimize the alcohol extraction process of Yigen Tiaoli paste based on entropy weight method weighted evaluation. Determination of psoralen and nootkatone by high performance liquid chromatography and determination of total flavonoids by UV spectrophotometry. The results showed that the weight coefficients of total flavonoids, psoralen and nootkatone were 0.336 9, 0.335 1 and 0.328 0, respectively. Optimization of extraction was as follows: extractions 2 times with 70% ethanol, and each time with 12-fold ethanol volume and 1h extraction. The extraction process is stable, and can be used as the alcohol extraction.

Key words: Yigen Tiaoli paste, total flavonoids, psoralen, nootkatone, alcohol extraction

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