湖北农业科学 ›› 2023, Vol. 62 ›› Issue (1): 157-161.doi: 10.14088/j.cnki.issn0439-8114.2023.01.027

• 检测分析 • 上一篇    下一篇

乙醇冷凝回流与超声波辅助提取葛根药材总黄酮

曾建光1, 徐雨生2, 徐翔1, 余正勇3, 金晨钟1   

  1. 1.湖南人文科技学院农业与生物技术学院,湖南 娄底 417000;
    2.湖南农业大学农学院,长沙 410128;
    3.文山学院三七医药学院,云南 文山 663000
  • 收稿日期:2022-09-05 出版日期:2023-01-25 发布日期:2023-03-07
  • 通讯作者: 金晨钟(1964-),男,教授,主要从事植物保护技术研究,(电子信箱)532479626@qq.com。
  • 作者简介:曾建光(1999-),男,湖南华容人,在读硕士研究生,研究方向为资源利用与植物保护,(电话)15581347964(电子信箱)zjg2315763483@163.com
  • 基金资助:
    湖南省高校创新平台开放基金项目(19K049)

Effects of ethanol condensation reflux extraction and ultrasonic-assisted extraction on the content of total flavonoids of Pueraria edulis

ZENG Jian-guang1, XU Yu-sheng2, XU Xiang1, YU Zheng-yong3, JIN Chen-zhong1   

  1. 1. School of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, Hunan, China;
    2. College of Agronomy, Hunan Agricultural University, Changsha 410128, China;
    3. College of Notoginseng Medicine and Pharmacy, Wenshan University, Wenshan 663000, Yunnan, China
  • Received:2022-09-05 Online:2023-01-25 Published:2023-03-07

摘要: 以云南省、广东省、广西壮族自治区不同地方葛根(Pueraria edulis)为材料,通过乙醇冷凝回流和超声波辅助提取法提取葛根药材中总黄酮,探究不同提取方式对不同地区葛根药材总黄酮含量的影响。结果表明,云南省葛根最佳提取时间均为20 min,广东省、广西壮族自治区葛根最佳提取时间均是30 min;冷凝回流提取法中葛根黄酮的得率相对较好;冷凝回流提取法中云南省产葛根总黄酮含量最高,平均含量为0.255 7%,广东省葛根总黄酮平均含量最低为0.210 8%。

关键词: 葛根(Pueraria edulis), 总黄酮, 冷凝回流提取法, 超声辅助提取法

Abstract: Taking Pueraria edulis from different places in Yunnan Province, Guangdong Province and Guangxi Zhuang autonomous region as materials, the medicinal materials of Pueraria edulis were extracted by ethanol condensation reflux extraction and ultrasonic-assisted extraction, and the effects of different extraction methods the contents of total flavonoids in Pueraria edulis from different areas were compared. The results showed that the best extraction time of Pueraria edulis in Yunnan Province was 20 min, and that in Guangdong and Guangxi Zhuang Autonomous Region was 30 min. The yield of total flavonoids from Pueraria edulis extracted by the condensation reflux extraction method was relatively better. The content of total flavonoids of Pueraria edulis in Yunnan was the highest with an average content of 0.255 7%, while the average content of total flavonoids in Pueraria edulis in Guangdong was the lowest with an average content of 0.210 8%.

Key words: Pueraria edulis, total flavonoids, condensation reflux extraction method, ultrasound-assisted extraction

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