两种小麦在盐胁迫下早期幼苗生长及生理生化指标变化规律

doi:10.14088/j.cnki.issn0439-8114.2021.15.005

摘要/Abstract

摘要： 研究了小麦耐盐品种新冬26和盐敏感品种鄂恩1号在盐胁迫下幼苗生长和相应生理生化指标的变化规律,为后续耐盐品种的选育提供依据。结果表明,在盐胁迫下,新冬26和鄂恩1号的根长、芽长、干重和鲜重随着盐浓度的增大而降低,而新冬26的相应指标比鄂恩1号下降幅度小。SOD、CAT、POD酶活性随着盐浓度的增加而增大,新冬26的酶活性高于鄂恩1号。在相同盐浓度下,随着盐胁迫时间的延长,酶活性呈现先上升后下降的趋势,新冬26的酶活性高于鄂恩1号;脯氨酸和MDA含量随着盐浓度的上升而增加,新冬26的脯氨酸含量高于鄂恩1号,MDA含量低于鄂恩1号;随着盐胁迫时间的延长,脯氨酸含量先上升后下降,而MDA持续上升,新冬26的脯氨酸含量最终高于鄂恩1号,MDA含量低于鄂恩1号。说明新冬26更能维持细胞稳态和细胞膜的稳定,表现更好的耐盐性。耐盐品种比敏感品种在盐胁迫下生长得更好,有较高的抗氧化酶活性,积累更多的脯氨酸来维持细胞稳态,而且耐盐品种的细胞膜受损程度较低,有更强的耐盐性。

关键词: 小麦, 盐胁迫, 抗氧化保护酶, 脯氨酸, MDA

Abstract: The changes of seedling growth and corresponding physiological and biochemical indexes of salt tolerant wheat variety Xindong 26 and salt sensitive wheat variety E'en 1 under salt stress were studied to provide basis for subsequent breeding of salt tolerant varieties. The results showed that under salt stress, the root length, bud length, dry weight and fresh weight of Xindong 26 and E'en 1 decreased with the increase of salt concentration, while the corresponding indexes of Xindong 26 decreased less than E'en 1; The activities of SOD, CAT and POD increased with the increase of salt concentration. The activity of Xindong 26 was higher than that of E'en 1. At the same concentration, the activity of SOD first increased and then decreased with the extension of stress time, but the activity of Xindong 26 was higher than that of E'en 1; The content of proline and MDA increased with the increase of salt concentration. The content of proline in Xindong 26 was higher than that in E'en 1, and the content of MDA was lower than that in E'en 1. At the same concentration, with the extension of stress time, the content of proline first increased and then decreased, while the content of MDA continued to rise. The content of proline in Xindong 26 was higher than that in E'en 1, and the content of MDA was lower than that in E'en 1, Xindong 26 could maintain cell homeostasis and cell membrane stability, and showed better salt tolerance. The salt tolerant varieties grew better than the sensitive varieties under salt stress, had higher antioxidant enzyme activities, accumulated more proline to maintain cell homeostasis, and the salt tolerant varieties had lower degree of cell membrane damage and stronger salt tolerance.

Key words: wheat, salt stress, antioxidant protective enzymes, proline, MDA

中图分类号:

S512.1⁺¹

肖冬宇, 吴端阳, 文雪, 周岚, 贺宏超, 王岩岩. 两种小麦在盐胁迫下早期幼苗生长及生理生化指标变化规律[J]. 湖北农业科学, 2021, 60(15): 29-33.

XIAO Dong-yu, WU Duan-yang, WEN Xue, ZHOU Lan, HE Hong-chao, WANG Yan-yan. Changes of early seedling growth and physiological and biochemical indexes of two wheat species under salt stress[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(15): 29-33.

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