十种芳香南药精油抑制真菌生长和毒素累积的效果比较

doi:10.14088/j.cnki.issn0439-8114.2024.07.017

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (7): 105-109.doi: 10.14088/j.cnki.issn0439-8114.2024.07.017

十种芳香南药精油抑制真菌生长和毒素累积的效果比较

梁焕宴, 邱淑芳, 冼梦婷, 周亚奎, 赵祥升

中国医学科学院药用植物研究所海南分所/海南省南药资源保护与开发重点实验室,海口 570311

收稿日期:2023-02-13 出版日期:2024-07-25 发布日期:2024-07-24
通讯作者: 赵祥升,（电话）0898-31589013（电子信箱）xiangsheng437@163.com。
作者简介:梁焕宴（2000-）,女,广东茂名人,在读硕士研究生,研究方向为中药安全性评价,（电话）18825950259（电子信箱）liang448594255@163.com
基金资助:
海南省重点研发项目（ZDYF2020183）; 国家自然科学基金项目（82074130）; 海南省自然科学基金人才项目（2019RC342）

Efficacy comparison of ten essential oils of aromatic southern medicines on the inhibition of fungal growth and mycotoxin accumulation

LIANG Huan-yan, QIU Shu-fang, XIAN Meng-ting, ZHOU Ya-kui, ZHAO Xiang-sheng

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine/Hainan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences , Haikou 570311, China

Received:2023-02-13 Published:2024-07-25 Online:2024-07-24

摘要/Abstract

摘要： 研究香茅[Cymbopogon nardus （L.） Rendle]等10种芳香南药精油对黄曲霉菌（Aspergillus flavus）、黑曲霉菌（Aspergillus niger）生长和黄曲霉毒素B₁、赭曲霉毒素A累积的抑制效果,以期将芳香南药精油开发为绿色、高效的杀菌剂。水蒸气蒸馏法提取精油,熏蒸法研究精油对黄曲霉菌和黑曲霉菌的抑制作用,气相色谱-质谱法分析精油成分,超高效液相色谱法检测黄曲霉毒素B₁和赭曲霉毒素A的含量。结果表明,10种精油的抑菌和抑制毒素累积作用存在较大差异。肉桂精油对黄曲霉菌、黑曲霉菌生长和毒素累积的抑制作用最强。香茅精油、大叶丁香精油、广藿香精油、降香精油对霉菌生长和毒素累积也有较强的抑制作用。益智精油、高良姜精油、白胡椒精油、莪术精油、沉香精油的抑菌效果不明显。甲基丁香酚、广藿香醇、柠檬醛、肉桂醛、反式橙花叔醇及其氧化物可能是发挥抗菌作用的成分。香茅、大叶丁香、广藿香、肉桂和降香精油具有开发为抑菌剂的潜力。

关键词: 芳香南药, 精油, 抑制能力, 黄曲霉菌, 黑曲霉菌, 真菌毒素

Abstract: The efficacy comparison of ten essential oils on the inhibition of fungal growth and mycotoxin accumulation was carried out to provide reference for the development of green and efficient bacteriostatic agents. The essential oils were extracted by steam distillation method and analyzed by gas chromatography-mass spectrometry （GC-MS）. Fumigation was used to test the inhibition on Aspergillus flavus, Aspergillus niger of essential oils. Aflatoxin B₁ （AFB₁） and ochratoxin A （OTA） were determined by ultrahigh-performance liquid chromatography-triple quadrupole tandem mass spectrometry （UPLC-MS/MS）. The results showed that, significant differences were found in the antifungal and antitoxin accumulation effects of ten essential oils. Cinnamon oil could completely inhibit the growth of A. flavus, A. niger and mycotoxins accumulation, while essential oils of Cymbopogon nardus, Dalbergia odorifera, Pogostemon cablin and Eugenia cayophyllata could inhibit the growth of the two fungi and mycotoxins. However, the antifungal effect of other essential oils was not significant. Methyl eugenol, patchouli alcohol, citral, cinnamaldehyde, trans-nerolidol and its oxides might be the components that exerted antifungal effects. Fragrance essential oils had the potential to be developed as green preservatives.

Key words: aromatic southern medicines, essential oils, antimicrobial activity, Aspergillus flavus, Aspergillus niger, mycotoxins

中图分类号:

S567
R932

梁焕宴, 邱淑芳, 冼梦婷, 周亚奎, 赵祥升. 十种芳香南药精油抑制真菌生长和毒素累积的效果比较[J]. 湖北农业科学, 2024, 63(7): 105-109.

LIANG Huan-yan, QIU Shu-fang, XIAN Meng-ting, ZHOU Ya-kui, ZHAO Xiang-sheng. Efficacy comparison of ten essential oils of aromatic southern medicines on the inhibition of fungal growth and mycotoxin accumulation[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(7): 105-109.

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十种芳香南药精油抑制真菌生长和毒素累积的效果比较

Efficacy comparison of ten essential oils of aromatic southern medicines on the inhibition of fungal growth and mycotoxin accumulation

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