关键词: 高葡萄糖, 诱导, 类二十烷酸代谢, 293T细胞

Abstract: 293T cells were induced with high glucose (50 mmol/L). The cell proliferation from days 1 to 4 was detected using a CCK-8 kit, and the mRNA expression levels of eicosanoid metabolism-related genes and inflammation-related genes were analyzed by real-time fluorescent quantitative PCR. The results showed that, compared with normal glucose (25 mmol/L), the mRNA expression levels of eicosanoid metabolism-related genes in 293T cells did not change significantly after high glucose stimulation. Only the COX1 gene exhibited transient downregulation at the 5th passage, which subsequently returned to normal levels. High glucose stimulation also significantly increased the mRNA level of the inflammatory factor IL-1β in 293T cells at the 11th passage, suggesting that long-term high glucose stimulation might induce mild inflammatory responses in 293T cells.

Key words: high glucose, induction, eicosanoid metabolism, 293T cells