小麦天冬酰胺合成酶基因家族鉴定及表达

doi:10.14088/j.cnki.issn0439-8114.2025.11.027

摘要/Abstract

摘要： 基于拟南芥和玉米天冬酰胺合成酶（ASN）基因家族的蛋白序列,利用生物信息学方法对小麦（Triticum aestivum L.）全基因组中的ASN基因家族进行系统鉴定,并对其成员的蛋白理化特性、保守结构域、染色体定位及系统进化关系进行综合分析;同时,结合转录组数据,系统解析ASN基因在小麦不同器官、发育阶段及生物与非生物胁迫条件下的表达模式。结果表明,14个小麦ASN基因家族成员均为亲水性蛋白,分布于11个染色体上;亚细胞定位预测表明,小麦ASN蛋白主要定位于细胞质,其次分布于细胞骨架和线粒体。系统进化分析结果表明,小麦ASN基因可被分3个亚家族,同一个亚家族成员间保守基序相似或一致。顺式作用元件分析发现,启动子区存在8类调控元件,主要包括脱落酸响应、赤霉素响应、干旱诱导、光响应及玉米醇溶蛋白代谢等元件,表明小麦ASN家族基因不仅参与多种逆境的应答反应,还参与多种激素调控。非生物胁迫分析表明,NaCl处理后,除TaASN11基因外,其他基因均表现为下调表达;PEG模拟干旱胁迫下,TaASN1、TaASN7、TaASN9基因表达下调,而TaASN8、TaASN10、TaASN11基因表达上调;ABA处理后,TaASN9基因表达下调,其他基因则表达上调。14个小麦ASN基因家族成员在进化上具有保守性,广泛参与生物和非生物逆境调控。

关键词: 小麦（Triticum aestivum L.）, 天冬酰胺合成酶（ASN）, 基因家族, 生物信息学, 鉴定, 表达

Abstract: Based on the protein sequences of the asparagine synthetase (ASN) gene family from Arabidopsis and maize, a genome-wide identification of the ASN gene family in wheat（Triticum aestivum L.） was conducted using bioinformatics methods, and a comprehensive analysis of the physicochemical properties, conserved domains, chromosomal locations, and phylogenetic relationships of its members was performed. Meanwhile, the expression patterns of ASN genes in different wheat organs, at various developmental stages, and under biotic and abiotic stresses were systematically analyzed using transcriptome data. The results showed that all 14 identified wheat ASN family members were hydrophilic proteins and distributed on 11 chromosomes. Subcellular localization prediction indicated that wheat ASN proteins were primarily located in the cytoplasm, followed by the cytoskeleton and mitochondria. Phylogenetic analysis revealed that the wheat ASN genes could be divided into three subfamilies, and the conserved motifs were similar or identical among members within the same subfamily. Cis-acting element analysis identified eight types of regulatory elements in the promoter regions, mainly including abscisic acid responsiveness, gibberellin responsiveness, drought inducibility, light responsiveness, and zein metabolism elements, suggesting that the wheat ASN family genes were involved not only in responses to various stresses but also in multiple hormone regulations. Analysis of abiotic stress responses showed that under NaCl treatment, the expression of all genes except TaASN11 was down-regulated. Under PEG-simulated drought stress, the expression of TaASN1, TaASN7, and TaASN9 was down-regulated, while that of TaASN8, TaASN10, and TaASN11 was up-regulated. After ABA treatment, the expression of TaASN9 was down-regulated, whereas the other genes were up-regulated. The 14 wheat ASN gene family members were evolutionarily conserved and widely involved in the regulation of biotic and abiotic stress responses.

Key words: wheat（Triticum aestivum L.）, asparagine synthetase（ASN）, gene family, bioinformatics, identification, expression

中图分类号:

S512

薛雨航, 刘易科, 宁强, 方正武. 小麦天冬酰胺合成酶基因家族鉴定及表达[J]. 湖北农业科学, 2025, 64(11): 195-202.

XUE Yu-hang, LIU Yi-ke, NING Qiang, FANG Zheng-wu. Genome-wide identification and expression analysis of the asparagine synthetase gene family in wheat[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(11): 195-202.

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