湖北农业科学 ›› 2025, Vol. 64 ›› Issue (12): 110-116.doi: 10.14088/j.cnki.issn0439-8114.2025.12.019

• 植物保护 • 上一篇    下一篇

云南根肿菌致病性差异评价

张顺荣1,2, 罗延青2, 刘亚俊3, 张云云2, 高金祥2, 张玉松2, 杨晖3, 程小毛1, 俎峰2   

  1. 1.西南林业大学园林园艺学院,昆明 650224;
    2.云南省草本油料遗传改良重点实验室/云南省农业科学院经济作物研究所,昆明 650225;
    3.临沧市农业技术推广站,云南 临沧 677099
  • 收稿日期:2025-07-07 发布日期:2025-12-30
  • 通讯作者: 程小毛(1979-),女,教授,博士,主要从事植物遗传育种研究,(电子信箱)xmcheng@swfu.edu.cn。
  • 作者简介:张顺荣(1997-),男,云南昆明人,在读硕士研究生,研究方向为油菜分子遗传育种,(电子信箱)ZSR13888727462@163.com;俎 峰(1984-),男,副研究员,博士,主要从事油菜分子遗传育种,(电子信箱)zf@yaas.org.cn。
  • 基金资助:
    云南省种子种业联合实验室项目(20205AR070001); 云南省兴滇英才支持计划项目(YNWR-QNBJ2019-280); 云南省创新人才培养对象项目(202305AD160025)

Evaluation of pathogenicity difference of Plasmodiophora brassicae in Yunnan

ZHANG Shun-rong1,2, LUO Yan-qing2, LIU Ya-jun3, ZHANG Yun-yun2, GAO Jin-xiang2, ZHANG Yu-song2, YANG Hui3, CHENG Xiao-mao1, ZU Feng2   

  1. 1. College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China;
    2. Key Laboratory of Herbaceous Oil Crop Genetic Improvement of Yunnan Province/Institute of Cash Crops, Yunnan Academy of Agricultural Sciences, Kunming 650225, China;
    3. Lincang Agricultural Technology Extension Station, Lincang 677099, Yunnan, China
  • Received:2025-07-07 Online:2025-12-30

摘要: 在优化根肿菌(Plasmodiophora brassicae Woronin)接种鉴定方法的基础上,选取5株云南省根肿病代表性菌株进行室内接种鉴定,以明确菌株间致病性差异。分别选取菊心大白菜(高感根肿病白菜品种)和华油杂62R(高抗根肿菌生理4号小种的甘蓝型油菜品种)为接种受体材料,通过分析不同菌株的发病率和病菌侵染率来评价其致病性差异。结果表明,不同菌株在菊心大白菜与华油杂62R上的侵染率存在显著差异,且所有菌株的侵染率均随调查时间推移显著上升,但2种寄主间不同菌株侵染率的差异趋势随接种时间延长表现出明显不同。

关键词: 根肿菌(Plasmodiophora brassicae Woronin), 接种方法, 致病性分析, 差异, 评价, 云南省

Abstract: Based on an optimized inoculation and identification method for Plasmodiophora brassicae Woronin, five representative strains of the clubroot pathogen from Yunnan Province were selected for indoor inoculation assays to clarify differences in pathogenicity among the strains. Two host materials, Juxin Chinese Cabbage (a highly clubroot-susceptible Chinese cabbage cultivar) and Huayouza 62R (a Brassica napus cultivar highly resistant to physiological race 4 of Plasmodiophora brassicae), were used as inoculation recipients. Pathogenicity differences among the strains were evaluated by analyzing disease incidence and pathogen infection rates. The results showed that infection rates of the different strains on both Juxin Chinese Cabbage and Huayouza 62R differed significantly. Furthermore, the infection rates of all strains increased significantly over the investigation period, but the variation trends in infection rates among strains showed clear differences between the two hosts as the inoculation period extended.

Key words: Plasmodiophora brassicae Woronin, inoculation method, pathogenicity analysis, difference, evaluation, Yunnan Province

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