滑子菇单孢菌株交配型鉴定及定向杂交育种

doi:10.14088/j.cnki.issn0439-8114.2026.10.015

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (10): 102-108.doi: 10.14088/j.cnki.issn0439-8114.2026.10.015

滑子菇单孢菌株交配型鉴定及定向杂交育种

王珺申¹, 贺妍², 刘文莹¹, 武喆喆¹, 贺晓龙^1,3, 刘博¹

1.延安大学生命科学学院,陕西延安 716000;
2.福建农林大学农学院,福州 350000;
3.陕西省黄土高原菌产业生态循环发展工程技术研究中心,陕西延安 716000

收稿日期:2026-05-25 出版日期:2026-10-25 发布日期:2026-10-23
通讯作者: 贺晓龙(1982-),男,陕西西安人,高级实验师,硕士,主要从事食药用菌种质资源开发、遗传育种及人工栽培与工厂化研究,(电子信箱)ydsky_mush@163.com。
作者简介:王珺申(1997-),男,陕西榆林人,在读硕士研究生,研究方向为食用菌育种,(电子信箱)15891783352@163.com
基金资助:
陕西省重点研发计划项目(2025NC-YBXM-078; 2024NC-YBXM-190; 2025NC-YBXM-270); 陕西省教育厅重点科学研究计划项目(24JR165); 陕西省科技创新团队项目(2023-CX-TD-40); 陕西省秦创原“科学家+工程师”队伍项目(2025QCY-KXJ-128); 延安市科技计划项目(2023-QYL-059; 2023-QYL-032; 2024-QYL-086; 2024-QYL-063); 延安大学研究生科研创新计划项目(YKY2026037)

Mating-type identification and directed hybridization breeding of single-spore strains of Pholiota nameko

WANG Jun-shen¹, HE Yan², LIU Wen-ying¹, WU Zhe-zhe¹, HE Xiao-long^1,3, LIU Bo¹

1. College of Life Sciences, Yan’an University, Yan’an 716000, Shaanxi, China;
2. College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350000, China;
3. Shaanxi Provincial Engineering and Technology Research Center for Ecological and Cyclic Development of the Edible Fungus Industry on the Loess Plateau,Yan’an 716000, Shaanxi, China

Received:2026-05-25 Published:2026-10-25 Online:2026-10-23

摘要/Abstract

摘要： 为筛选具有潜在育种价值的滑子菇(Pholiota nameko)杂交菌株,以鳞伞属6株近缘菌株为试验材料,采用组织分离法获得纯培养菌丝,基于ITS序列进行分子鉴定与系统发育分析,同时利用交配型A位点特异性引物,通过PCR检测亲本及单孢菌株的交配型基因类型,并开展不同交配型单孢菌株两两配对杂交,结合拮抗试验对杂交菌株进行初步筛选。结果表明,6株供试菌株分属3个物种,其中G6为光帽鳞伞(Pholiota nameko),G4为柠檬鳞伞(Pholiota limonella),G1、G2、G3、G5均为小孢鳞伞(Pholiota microspora),不同菌株间在系统发育关系上表现出一定分化。共检测出A1、A2、A3、A4、A5、A6共6种A交配型因子类型,6株亲本菌株G1至G6的交配型依次为A1A2、A2A4、A1A4、A3A5、A5A6、A3A4。通过单×单杂交获得112个组合,最终筛选出18株遗传性状稳定的候选杂交菌株。

关键词: 滑子菇(Pholiota nameko), 单孢菌株, 交配型, 分子标记, 定向杂交育种

Abstract: To screen for Pholiota nameko hybrid strains with potential breeding value, six closely related strains of the genus Pholiota were used as experimental materials. Pure mycelial cultures were obtained by the tissue isolation method, and molecular identification and phylogenetic analysis were performed based on ITS (internal transcribed spacer) sequences. In addition, specific primers targeting the A mating-type locus were used for PCR amplification to detect the mating-type genes of the parental strains and their monospore isolates. Subsequently, monospore isolates with different mating types were paired for cross-hybridization, and the resulting hybrid strains were preliminarily screened by antagonism tests. The results showed that the six tested strains were classified into three species. Specifically, strain G6 was identified as Pholiota nameko, strain G4 as Pholiota limonella, and strains G1, G2, G3, and G5 as Pholiota microspora, with certain phylogenetic differentiation observed among the tested strains. A total of six A mating-type factors, namely A1, A2, A3, A4, A5, and A6, were detected among the tested strains, and the mating-type genotypes of the six parental strains G1-G6 were A1A2, A2A4, A1A4, A3A5, A5A6, and A3A4, respectively. A total of 112 cross combinations were obtained through monospore × monospore hybridization, and 18 candidate hybrid strains with stable genetic traits were finally selected through antagonism testing.

Key words: Pholiota nameko, monospore isolates, mating type, molecular markers, directed hybridization breeding

中图分类号:

S646.1

王珺申, 贺妍, 刘文莹, 武喆喆, 贺晓龙, 刘博. 滑子菇单孢菌株交配型鉴定及定向杂交育种[J]. 湖北农业科学, 2026, 65(10): 102-108.

WANG Jun-shen, HE Yan, LIU Wen-ying, WU Zhe-zhe, HE Xiao-long, LIU Bo. Mating-type identification and directed hybridization breeding of single-spore strains of Pholiota nameko[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(10): 102-108.

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滑子菇单孢菌株交配型鉴定及定向杂交育种

Mating-type identification and directed hybridization breeding of single-spore strains of Pholiota nameko

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