Distinctive Nuclease Cas13a： The New CRISPR Platform for Editing RNAs and Its Progress

doi:10.14088/j.cnki.issn0439-8114.2018.02.001

Abstract

Abstract: The CRISPR（clustered regularly interspaced short palindromic repeats） platform is an excellent genome editing platform, in which, the novel nuclease Cas13a （CRISPR-associated protein 13a） can edit DNA and RNA. This new platform could fulfill DNA or RNA detection with molar sensitivity and single-base mismatch specificity, and meanwhile be extended to fine detect of viruses（even Close relatives of Zika and Dengue strains） and bacteria, monitor early cancer, with the simple technical manipulation and a wide range of application potentials, which could be another excellent tool of genomic editing. Here the current progress about Cas13a system was overviewed.

Key words: Genomic Editing, CRISPR, CRISPR-associated protein 13a, RNA, gene network regulation

CLC Number:

S188

LIU Gui-sheng, MEKCNAY Supamit, WU Jun-jing, QIAO Mu, PENG Xian-wen, MEI Shu-qi. Distinctive Nuclease Cas13a： The New CRISPR Platform for Editing RNAs and Its Progress[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(2): 5-8.

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