Optimizing on Fermentation Conditions of Bacillus subtilis by Response Surface Analysis Methodology

doi:10.14088/j.cnki.issn0439-8114.2018.21.033

HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (21): 127-129.doi: 10.14088/j.cnki.issn0439-8114.2018.21.033

• Biological Engineering • Previous Articles Next Articles

Optimizing on Fermentation Conditions of Bacillus subtilis by Response Surface Analysis Methodology

RAO Ben, ZHOU Rong-hua, MIN Yong, LIAO Xian-qing, LIU Fang, CHEN Wei, ZHANG Guang-yang

National Biopesticide Engineering Technology Research Center,Wuhan 430064,China

Received:2018-08-14 Online:2018-11-10 Published:2020-01-13

Abstract

Abstract: In order to obtain the optimal medium composition for Bacillus subtilis production,the Plackett-Burman design method was used to evaluate the effects of five variables,and the optimum levels of key factors affecting spore production were conducted by response surface analysis methodology. The results showed that,the optimal values of the two parameters determined by response surface analysis methodology were corn starch 2.48 g/mL and corn-soybean 4.90 g/mL. The predicted spore production was 1.05×10¹⁰ CFU/mL,and the actual experimental result was 1.10×10¹⁰ CFU/mL,they were in agreement and it is 1.37 folds of the production before optimization （8×10⁹ CFU/mL）.

Key words: Bacillus subtilis, Plackett-Burman design method, response surface analysis methodology

CLC Number:

Q813.1

RAO Ben, ZHOU Rong-hua, MIN Yong, LIAO Xian-qing, LIU Fang, CHEN Wei, ZHANG Guang-yang. Optimizing on Fermentation Conditions of Bacillus subtilis by Response Surface Analysis Methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(21): 127-129.

References 9

[1]	朱晓立,俞巍蔚.枯草芽孢杆菌产芽孢发酵培养基的优化研究[J].化工设计通讯,2016,42(6):105-106.
[2]	陈静宇,李晶,张淑梅,等.枯草芽孢杆菌B91产芽孢发酵条件优化[J].黑龙江科学,2016,7(12):1-3.
[3]	李卓伟,杨国辉,王德功,等.枯草芽孢杆菌YZ-1菌株的产芽孢发酵条件优化[J].黑龙江畜牧兽医,2015(21):153-156.
[4]	王金玲,赵凤艳,吕长山,等.胶质芽孢杆菌液体发酵产孢培养基的优化[J].食品与生物技术学报,2013,32(4):417-424.
[5]	甄静,郭直岳,谢宝恩,等.枯草芽孢杆菌XK-1产芽孢条件的优化[J].中国农学通报,2012,28(27):146-151.
[6]	郭夏丽,狄源宁,王岩.枯草芽孢杆菌产芽孢条件的优化[J].中国土壤与肥料,2012(3):99-103.
[7]	张俊会,马国芳.光学显微镜下细菌芽孢染色方法的探讨[J].生物学杂志,2011,28(6):106-108.
[8]	陈秋红,孙梅,匡群,等.培养条件对凝结芽孢杆菌芽孢形成的影响[J].生物技术,2009,19(1):77-81.
[9]	任香芸,蔡海松,林新坚,等.嗜热脂肪土芽孢杆菌CHB1发酵培养基的优化[J].福建农业学报,2007,22(1):54-57.

Optimizing on Fermentation Conditions of Bacillus subtilis by Response Surface Analysis Methodology

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